# J659: PELAGICOCCUS AGAR


## Main sol. J659

55.1	g	Marine agar 2216 (BD-Difco)	
9.1	g	R2A agar (BD-Difco)	
750	ml	**Artificial seawater**	
250	ml	Distilled water	


## Artificial seawater

24	g	NaCl	
7	g	MgSO4 x 7 H2O	
5.3	g	MgCl2 x 6 H2O	
0.7	g	KCl	
0.1	g	CaCl2 x 2 H2O	
1000	ml	Distilled water	

1. Adjust pH to 7.5.
2. Comment: For preparation of the liquid medium, use Marine broth 2216 (BD-Difco) instead of Marine agar 2216 and R2A broth instead of R2A agar by omitting agar from the R2A agar medium composition (see Medium No. 346).