# J672: FLEXIBACTER CANADENSIS MEDIUM


## Main sol. J672

0.1	g	MgSO4 x 7 H2O	
0.1	g	KNO3	
0.1	g	CaCl2 x 2 H2O	
0.1	g	Sodium glycerophosphate	
1	ml	**Trace element solution SL-10**	
1	g	Tris(hydroxymethyl)aminomethane	
1	mg	Thiamine HCl	
1	g	Casamino acids (BD-Difco)	
1	g	Glucose	
1	�g	Vitamin B12	
1000	ml	Distilled water	

1. Adjust pH to 7.5.


## Trace element solution SL-10

10	ml	HCl (25%, 7.7 M)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.