# J707: LACTATE SULFATE MEDIUM


## Main sol. J707

1	g	Yeast extract	
7.1	g	Na2SO4	
6.95	g	L-Sodium lactate	
1.97	g	MgSO4 x 7 H2O	
1.08	g	NH4Cl	
0.38	g	K2HPO4	
0.09	g	CaCl2 x 2 H2O	
2	ml	**Trace vitamins**	
0.2	ml	Coenzyme M (0.01%)	
0.1	ml	**Vitamin B12 solution 0.05 g/L**	
12.5	ml	**Trace element solution**	
5.96	g	HEPES	
1	mg	Resazurin	
1000	ml	Distilled water	

1. Autoclave under a N2 gas atmosphere. After cooling, add the following sterile solutions (autoclaved or *filter-sterilized): 

10	ml	NaHCO3 (*, 7%)	
10	ml	L-Cysteine HCl x H2O (2.5%)	

2. Adjust pH to 7.2.


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water	


## Vitamin B12 solution 0.05 g/L

5	mg	Vitamin B12	
100	ml	Distilled water	


## Trace element solution

12.8	g	Nitrilotriacetic acid	
0.42	g	FeSO4 x 7 H2O	
0.01	g	H3BO3	
0.1	g	MnCl2 x 4 H2O	
0.17	g	CoCl2 x 6 H2O	
0.02	g	CuCl2 x 2 H2O	
0.21	g	ZnSO4 x 7 H2O	
0.01	g	Na2MoO4 x 2 H2O	

1. Dissolve nitrilotriacetic acid in 800 ml distilled water and adjust pH to 6.5 with NaOH. Then proceed to add the minerals and bring volume to 1.0 L.