# J781: CLOSTRIDIUM ACETATE 1 MEDIUM


## Main sol. J781

0.3	g	KH2PO4	
0.3	g	K2HPO4	
1	g	NH4Cl	
1	g	NaCl	
0.1	g	KCl	
0.1	g	CaCl2 x 2 H2O	
0.5	g	MgCl2 x 6 H2O	
10	ml	**Trace element solution**	
1	g	Yeast extract	
0.5	g	L-Cysteine HCl x H2O	
1	mg	Resazurin	
1000	ml	Distilled water	

1. Mix components thoroughly, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): 

25	ml	NaHCO3 (*, 8%)	
20	ml	Fructose (1 M)	
8	ml	Na2S x 9 H2O (5%)	


## Trace element solution

12.5	ml	HCl (25%)	
2.1	g	FeSO4 x 7 H2O	
30	mg	H3BO3	
100	mg	MnCl2 x 4 H2O	
190	mg	CoCl2 x 6 H2O	
24	mg	NiCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
144	mg	ZnSO4 x 7 H2O	
36	mg	Na2MoO4 x 2 H2O	
987	ml	Distilled water