# J830: DESULFOVIBRIO B0109P2 MEDIUM


## Main sol. J830

1	g	NH4Cl	
0.3	g	KH2PO4	
0.3	g	K2HPO4	
2.8	g	Na2SO4	
20	g	NaCl	
0.1	g	KCl	
0.1	g	CaCl2 x 2 H2O	
3.16	g	Na2S2O3 x 5 H2O	
0.1	g	Yeast extract	
1	ml	**Trace element solution**	
0.5	g	L-Cysteine HCl x H2O	
1	mg	Resazurin	
900	ml	Distilled water	

1. To complete the medium, aseptically and anaerobically add the following stock solutions (per liter, final volume): 

20	ml	Sodium lactate (1.0 M)	
20	ml	MgCl2 x 6 H2O (15%)	
40	ml	NaHCO3 (5%)	
20	ml	Na2S x 9 H2O (2%)	

2. Mix ingredients and adjust pH to 7.4. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture tubes under the same gas mixture, seal with butyl rubber stoppers and autoclave.
3. Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere.
4. Readjust pH of the medium to 7.4, if necessary.


## Trace element solution

12.5	ml	HCl (25%)	
2.1	g	FeSO4 x 7 H2O	
30	mg	H3BO3	
100	mg	MnCl2 x 4 H2O	
190	mg	CoCl2 x 6 H2O	
24	mg	NiCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
144	mg	ZnSO4 x 7 H2O	
36	mg	Na2MoO4 x 2 H2O	
987	ml	Distilled water