# J865: DESULFOSPOROSINUS BSREI1 MEDIUM ## Main sol. J865 0.45 g (NH4)2SO4 0.05 g KH2PO4 0.5 g MgSO4 x 7 H2O 0.05 g KCl 0.014 g Ca(NO3)2 x 4 H2O 3 g Na2SO4 1 ml **Trace element solution** 0.5 g Yeast extract 0.5 g L-Cysteine HCl x H2O 1 mg Resazurin 910 ml Distilled water 1. Mix components thoroughly, distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 910 ml medium) from anaerobic stocks (autoclaved or *filter-sterilized): 60 ml NaHCO3 (*, 5%) 20 ml Fructose (1 M) 10 ml Trace vitamins (see Medium No. 197) (*) 8 ml Na2S x 9 H2O (5%) ## Trace element solution 12.5 ml HCl (25%) 2.1 g FeSO4 x 7 H2O 30 mg H3BO3 100 mg MnCl2 x 4 H2O 190 mg CoCl2 x 6 H2O 24 mg NiCl2 x 6 H2O 2 mg CuCl2 x 2 H2O 144 mg ZnSO4 x 7 H2O 36 mg Na2MoO4 x 2 H2O 987 ml Distilled water