# J88: 1% OGAWA MEDIUM ## Main sol. J88 ## Solution I 10 g Na glutamate 10 g KH2PO4 1000 ml Distilled water ## Solution II 6 ml Glycerol 6 ml Malachite green (2%) 200 ml Whole egg 1. Homogenize Solution II by stirring and filter through gauze. Mix solutions I and II, and sterilize at 90C for 60 min. 2. Comment: Commercial 1% Ogawa medium (Eiken or Nissui) is also available.