# J899: THERMOBACTER ROSEUM MEDIUM


## Main sol. J899

0.2	g	KH2PO4	
0.25	g	NH4Cl	
1	g	NaCl	
0.4	g	MgCl2 x 6 H2O	
0.5	g	KCl	
0.1	g	CaCl2 x 2 H2O	
0.1	g	NaHCO3	
1	mg	Resazurin	
2	g	Yeast extract (BD-Difco)	
1000	ml	Distilled water	

1. Mix components thoroughly and autoclave under a N2 gas atmosphere. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): 

1	ml	**Vitamin solution** (*)	
1	ml	**Trace element solution** (*)	
6	ml	Na2S x 9 H2O (5%)	

2. Check pH to be 7.5 - 7.6.


## Vitamin solution

25	mg	p-Aminobenzoic acid	
100	mg	Biotin	
25	mg	Nicotinic acid	
25	mg	DL-Calcium pantothenate	
25	mg	Pyridoxine hydrochloride	
10	mg	Folic acid	
25	mg	Riboflavin	
0.5	mg	Vitamin B12	
25	mg	Lipoic acid	
1000	ml	Distilled water	


## Trace element solution

784	mg	Fe(NH4)2(SO4)2 x 6 H2O	
238	mg	CoCl2 x 6 H2O	
395	mg	(NH4)2Ni(SO4)2 x 6 H2O	
24	mg	Na2MoO4 x 2 H2O	
33	mg	Na2WO4 x 2 H2O	
144	mg	ZnSO4 x 7 H2O	
2	mg	CuCl2 x 2 H2O	
94	mg	Na2SeO4	
6	mg	H3BO3	
99	mg	MnCl2 x 4 H2O	
1000	ml	Distilled water