# J935: FERVIDICOCCUS FONTIS MEDIUM ## Main sol. J935 0.33 g NH4Cl 0.33 g KH2PO4 0.33 g KCl 0.44 g CaCl2 x 2 H2O 0.7 g MgCl2 x 6 H2O 0.5 g NaCl 0.8 g NaHCO3 1 ml **FeCl2 solution** 1 ml **Trace element solution** 1 mg Resazurin 1000 ml Distilled water 1. Mix components thoroughly, adjust pH to 5.0 and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): 5 ml (w/v) Yeast extract (BD-Difco, 10%) 20 ml (w/v) Trypticase peptone (BD-BBL, 10%) 10 ml **Trace vitamins** (*) 10 ml Na2S x 9 H2O (w/v, 5%) 2. Readjust pH to 5.5 - 6.0 with sterile 1 N H2SO4, if necessary. ## FeCl2 solution 10 ml HCl (7.7 M, 25%) 1.5 g FeCl2 x 4 H2O 990 ml Distilled water ## Trace element solution 70 mg ZnCl2 100 mg MnCl2 x 4 H2O 6 mg H3BO3 190 mg CoCl2 x 6 H2O 2 mg CuCl2 x 2 H2O 24 mg NiCl2 x 6 H2O 36 mg Na2MoO4 x 2 H2O 1000 ml Distilled water ## Trace vitamins 2 mg Biotin 2 mg Folic acid 10 mg Pyridoxine hydrochloride 5 mg Thiamine HCl 5 mg Riboflavin 5 mg Nicotinic acid 5 mg Calcium pantothenate 0.1 mg Vitamin B12 5 mg p-Aminobenzoic acid 5 mg Lipoic acid 1000 ml Distilled water