# J940: AMINIVIBRIO ASINOENSIS MEDIUM


## Main sol. J940

0.2	g	KH2PO4	
0.25	g	NH4Cl	
1	g	NaCl	
0.5	g	KCl	
0.4	g	MgCl2 x 6 H2O	
0.15	g	CaCl2 x 2 H2O	
1.1	g	Sodium pyruvate	
2	g	Yeast extract	
1	ml	**FeCl2 solution**	
1	ml	**Trace element solution**	
1	ml	**Selenite-tungstate solution**	
0.4	mg	Resazurin	
1000	ml	Distilled water	

1. Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (filter-sterilized): 

30	ml	NaHCO3 (8%)	
1	ml	**Vitamin solution**	

2. Distribute the medium into culture vessels under the same gas mixture. Finally, add per liter the following solution from an anaerobic stock: 

7	ml	Na2S x 9 H2O (5%)	


## Vitamin solution

80	mg	p-Aminobenzoic acid	
20	mg	Biotin	
200	mg	Thiamine HCl	
50	mg	Vitamin B12	
1000	ml	Distilled water	


## FeCl2 solution

10	ml	HCl (7.7 M, 25%)	
1.5	g	FeCl2 x 4 H2O	
990	ml	Distilled water	


## Trace element solution

70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	


## Selenite-tungstate solution

0.4	g	NaOH	
6	mg	Na2SeO3 x 5 H2O	
8	mg	Na2WO4 x 2 H2O	
1000	ml	Distilled water