# J974: ALKALINUS ABYSSINIESE MEDIUM


## Main sol. J974

117	g	NaCl	
5	g	Yeast extract (BD-Difco)	
5	g	Peptone (Oxoid)	
0.2	g	MgSO4 x 7 H2O	
0.2	g	CaCl2 x 2 H2O	
0.1	g	KH2PO4	
10	ml	**Trace mineral solution**	

1. Add components to distilled water and bring volume to 900 ml. For preparation of solid medium, add 20.0 g/L agar. Autoclave and cool (to 50 - 60C for agar medium). Then, add aseptically the following solutions (separately autoclaved): 

5	ml	Glucose (w/v, 10%)	
100	ml	Na2CO3 (w/v, 10%)	


## Trace mineral solution

30	mg	H3BO4	
10	mg	ZnSO4 x 7 H2O	
3	mg	Na2MoO4 x 2 H2O	
3	mg	MnCl2 x 4 H2O	
2	mg	NiCl2 x 6 H2O	
10	mg	CoCl2 x 6 H2O	
1	mg	CuCl2 x 2 H2O	
1000	ml	Distilled water