# J975: SALINIVIBRIO SHARMENSIS MEDIUM ## Main sol. J975 100 g NaCl 3 g Trisodium citrate 1 g MgSO4 x 7 H2O 2 g KCl 0.36 mg MnCl2 x 4 H2O 0.05 g FeSO4 x 7 H2O 10 g Yeast extract (BD-Difco) 1. Add components to distilled water and bring volume to 970 ml. For preparation of solid medium, add 20.0 g/L agar. Autoclave and cool (to 50 - 60C for agar medium). Then, add the following solution (separately autoclaved): 30 ml Na2CO3 (w/v, 10%) 2. Check the final pH to be around 9.0.