1183 .
ROSEICYCLUS MEDIUM
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Selected strain: Roseicyclus mahoneyensis DSM 16097
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Equipment needed:
Autoclave
Filter
Final pH:
8.0 - 8.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KCl | 0.3 | g | ||
|
|
NH4Cl | 0.3 | g | ||
|
|
MgSO4 | 2.0 | g | ||
|
|
Na2SO4 | 15.0 | g | ||
|
|
Malic acid | 1.0 | g | ||
|
|
Na acetate | 1.0 | g | ||
|
|
Yeast extract | 1.0 | g | ||
|
|
Bacto peptone | 0.5 | g | ||
|
|
Trace elements solution | 2.0 | ml | ||
|
|
Vitamin solution | 2.0 | ml | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Add to the autoclaved medium the following, from sterile stock solutions | |||||
|
|
K2HPO4
(10%) |
3.0 | ml | ||
|
|
NaHCO3
(10%) |
5.0 | ml | ||
|
|
CaCl2
(10%) |
0.5 | ml | ||
| 2 pH is about 5.9 before autoclaving. | |||||
| 3 The pH of the final medium should be 8.0-8.5. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nicotinic acid | 400.0 | mg | ||
|
|
Thiamine | 400.0 | mg | ||
|
|
Biotin | 80.0 | mg | ||
|
|
Vitamin B12 | 0.5 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Filter sterilise | |||||
Trace elements solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
FeSO4 x 7 H2O | 300 | mg | ||
|
|
ZnSO4 x 7 H2O | 5 | mg | ||
|
|
MnCl2 x 4 H2O | 3 | mg | ||
|
|
H3BO3 | 2 | mg | ||
|
|
CoCl2 x 6 H2O | 1 | mg | ||
|
|
CuCl2 x 2 H2O | 2 | mg | ||
|
|
NiCl2 x 6 H2O | 3 | mg | ||
|
|
Na2MoO4 x 2 H2O | 3 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust the pH to 3.0 with HCl and filter sterilise, store at 4°C. | |||||