1396 .
HALOARCHAEAL MEDIUM MH-1
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* Medium for Halarchaeum acidiphilum DSM 22442
The MgCl2 x 6H2O concentration of strain MH1-52-1 (DSM 22442) for well growth is 10.0 mM (20.0 g/l).
Growth conditions: 37 °C
Equipment needed:
Final pH:
4.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
L-Glutamic acid | 2 | g | ||
|
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Trisodium citrate x 2 H2O | 2 | g | ||
|
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KH2PO4 | 1 | g | ||
|
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NH4Cl | 1 | g | ||
|
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K2SO4 | 5 | g | ||
|
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NaCl | 200 | g | ||
|
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Casamino acids | 4 | g | ||
|
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Yeast extract | 2 | g | ||
|
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FeSO4 x 6 H2O | 4 | mg | ||
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Trace Element Solution | 2 | ml | ||
|
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Agar | 20 | g | ||
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MgCl2 x 6 H2O | 20 | g/l | ||
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Distilled water | 800 | ml | ||
| 1 Adjust to pH 4.5 with 40% KOH and sterilize by autoclaving. | |||||
Trace Element Solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
CoCl2 x 6 H2O | 0.30 | g | ||
|
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MnCl2 x 4 H2O | 0.10 | g | ||
|
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ZnCl2 | 0.10 | g | ||
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H3BO4 | 0.02 | g | ||
|
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Na2MoO4 x 2 H2O | 0.10 | g | ||
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NiCl2 x 6 H2O | 0.10 | g | ||
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CuCl2 x 6 H2O | 0.01 | g | ||
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AlCl3 | 0.04 | g | ||
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Na2WO4 x 2 H2O | 0.02 | g | ||
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CaCl2 x 2 H2O | 1.00 | g | ||
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BaCl2 x 2 H2O | 0.01 | g | ||
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Na2S2O3 x 5 H2O | 2.00 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Adjust to pH 4.0. | |||||