1 .
NUTRIENT AGAR
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* Medium for Dickeya paradisiaca DSM 18069
Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1 or 535. Subsequent subculturing may be carried out in liquid medium or on agar plates
Growth conditions: 2-3 days
aerobic
30 °C
Equipment needed:
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone | 5 | g | ||
|
|
Meat extract | 3 | g | ||
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Agar , for solid medium | 15 | g | ||
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Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.0. | |||||
| 2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||