1 .
NUTRIENT AGAR
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* Medium for Methylopila oligotropha DSM 29295
Plus 5 g/l NaCl plus 1 % (v/v) MeOH
Growth conditions: 28 °C
Equipment needed:
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone | 5 | g | ||
|
|
Meat extract | 3 | g | ||
|
|
Agar , for solid medium | 15 | g | ||
|
|
NaCl | 5 | g/l | ||
|
|
Methanol | 1 | %(v/v) | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.0. | |||||
| 2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||