Plus 5 g/l NaCl; Reactivate and grow freeze-dried cells from the ampoule in liquid medium 1 or 365. Subsequent subculturing may be carried out in liquid medium or on agar.
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Peptone | 5 | g | ||
|
Meat extract | 3 | g | ||
|
Agar , for solid medium | 15 | g | ||
|
NaCl | 5 | g/l | ||
|
Distilled water | 1000 | ml | ||
1 Adjust pH to 7.0. | |||||
2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. |