1a .
REACTIVATION WITH LIQUID MEDIUM 1
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* Medium for Chitiniphilus shinanonensis DSM 23277
Plus 5 g/l NaCl
Equipment needed:
Final pH:
7.0
Select a strain (optional):
ml
| 1 Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates. | |||||
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Peptone | 5 | g | ||
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Meat extract | 3 | g | ||
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Agar , for solid medium | 15 | g | ||
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NaCl | 5 | g/l | ||
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Distilled water | 1000 | ml | ||
| 2 Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||