add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).
Compound | Amount | Unit | |
---|---|---|---|
|
Brain heart infusion
(Difco) |
37.00 | g |
|
DTT | 0.02 | g/ml |
|
Cysteine | 0.05 | g/ml |
|
Distilled water | 1000.00 | ml |