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COLUMBIA BLOOD AGAR
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* Medium for Alysiella crassa DSM 2578
Add enough liquid medium 220, 535 or 215 to the agar plate when inoculating. The surfaces of agar plates must be moist during the whole incubation period. Inoculate with 1 ml cell suspension.
Growth conditions: 37 °C
Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Columbia agar base | 1000 | ml | ||
|
|
Horse blood | 4 | % | ||
| 1 Columbia agar base supplemented with 4% horse blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below). For rehydration of Helicobacter and Campylobacter spp., use medium 215 (brain heart infusion). | |||||
545. TRYPTONE SOYA BROTH (TSB) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone | 17.0 | g | ||
|
|
Soy peptone | 3.0 | g | ||
|
|
D(+)-Glucose | 2.5 | g | ||
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NaCl | 5.0 | g | ||
|
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K2HPO4 | 2.5 | g | ||
|
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Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 7.3. | |||||
| 2 The same as Caso Bouillon (Merck or Oxoid, Germany). | |||||
215. BHI MEDIUM #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Brain heart infusion
(Difco) |
37 | g |
|
|
Distilled water | 1000 | ml |
1. NUTRIENT BROTH #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone | 5 | g | ||
|
|
Meat extract | 3 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.0. | |||||