Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth. Subsequent subculturing may be carried out in liquid medium or on agar plates
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Tryptone | 5.0 | g | ||
|
Yeast extract | 2.5 | g | ||
|
Dextrose | 1.0 | g | ||
|
Agar | 15.0 | g | ||
|
Distilled water | 1000.0 | ml | ||
1 Adjust pH to 7.0. |