465h .
MINERALMEDIUM WITH 6-METHYLNICOTINATE
What is this page?
Selected strain: Mesorhizobium sp. DSM 11455
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Equipment needed:
Autoclave
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Medium 465 | 1000 | ml | ||
|
|
Selenite/tungstate stock solution | 1 | ml | ||
|
|
6-methylnicotinate , adjust if required | 5 | g/l | ||
|
|
Vitamin solution | 1 | ml | ||
| 1 Supplement medium 465 with selenite, tungstate, 2.5 - 5 g/l 6-methylnicotinate from a stock solution (autoclave separately) and 1 ml/l vitamin solution (see medium 461). | |||||
Main sol. 465 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2HPO4 x 2 H2O | 3.50 | g | ||
|
|
KH2PO4 | 1.00 | g | ||
|
|
(NH4)2SO4 | 0.50 | g | ||
|
|
MgCl2 x 6 H2O | 0.10 | g | ||
|
|
Ca(NO3)2 x 4 H2O | 0.05 | g | ||
|
|
Trace element solution SL-4 | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 pH 7.25 | |||||
| 2 Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source. | |||||
Selenite/tungstate stock solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaOH | 500 | mg | ||
|
|
Na2SeO3 x 5 H2O | 3 | mg | ||
|
|
Na2WO4 x 2 H2O | 4 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Autoclave separately. Add 1 ml per l medium. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Vitamin B12 | 50 | mg | ||
|
|
Pantothenic acid | 50 | mg | ||
|
|
Riboflavin | 50 | mg | ||
|
|
Pyridoxamine hydrochloride | 10 | mg | ||
|
|
Biotin | 20 | mg | ||
|
|
Folic acid | 20 | mg | ||
|
|
Nicotinic acid | 25 | mg | ||
|
|
Nicotine amide | 25 | mg | ||
|
|
alpha-lipoic acid | 50 | mg | ||
|
|
p-Aminobenzoic acid | 50 | mg | ||
|
|
Thiamine-HCl x 2 H2O | 50 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Stir for some hours, filter sterilize the solution. | |||||
Trace element solution SL-4 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 0.50 | g | ||
|
|
FeSO4 x 7 H2O | 0.20 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
MnCl2 x 4 H2O | 0.03 | g | ||
|
|
H3BO3 | 0.30 | g | ||
|
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 2 H2O | 0.01 | g | ||
|
|
NiCl2 x 6 H2O | 0.02 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. | |||||
Main sol. 1 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone | 5 | g | ||
|
|
Meat extract | 3 | g | ||
|
|
Agar , for solid medium | 15 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.0. | |||||
| 2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||
Main sol. 220 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone | 15 | g | ||
|
|
Soy peptone | 5 | g | ||
|
|
NaCl | 5 | g | ||
|
|
Agar | 15 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131). | |||||
Main sol. 535 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase soy broth
(BBL 11768, Oxoid CM129 or Merck 5459) |
30 | g | ||
|
|
Agar | 20 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 pH 7.3 | |||||
| 2 Autoclave at 121°C for 15 min. | |||||
Main sol. 830 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 0.50 | g | ||
|
|
Proteose peptone
(Difco no. 3) |
0.50 | g | ||
|
|
Casamino acids | 0.50 | g | ||
|
|
Glucose | 0.50 | g | ||
|
|
Starch
(soluble) |
0.50 | g | ||
|
|
Na-pyruvate | 0.30 | g | ||
|
|
K2HPO4 | 0.30 | g | ||
|
|
MgSO4 x 7 H2O | 0.05 | g | ||
|
|
Agar | 15.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C. | |||||