486 .
THIOBACILLUS THIOPARUS (TK-m) MEDIUM
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Selected strain: Thiobacillus thioparus DSM 5368
This strain uses the standard medium without modifications.
Growth conditions: 2-3 days
30 °C
Equipment needed:
Autoclave
Filter
Final pH:
7.1
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 2.0 | g | ||
|
|
K2HPO4 | 2.0 | g | ||
|
|
NH4Cl | 0.4 | g | ||
|
|
Na2CO3 | 0.4 | g | ||
|
|
MgCl2 x 6 H2O | 0.2 | g | ||
|
|
Vitamin solution | 3.0 | ml | ||
|
|
Trace element solution | 1.0 | ml | ||
|
|
Bromocresol purple
(saturated aq. solution) |
2.0 | ml | ||
|
|
Na2S2O3 x 5 H2O | 5.0 | g | ||
|
|
Agar | 15.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Sterilize the phosphates separately in 1/10 of medium volume and mix with the other salts when cool. Add filter-sterilized vitamin solution after autoclaving to sterile medium. Adjust pH of complete medium to 7.1, if necessary. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Thiamine-HCl x 2 H2O | 10 | mg | ||
|
|
Nicotinic acid | 20 | mg | ||
|
|
Pyridoxine hydrochloride | 20 | mg | ||
|
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p-Aminobenzoic acid | 10 | mg | ||
|
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Riboflavin | 20 | mg | ||
|
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Calcium pantothenate | 20 | mg | ||
|
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Biotin | 1 | mg | ||
|
|
Vitamin B12 | 1 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.0. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 50.00 | g | ||
|
|
ZnSO4 x 7 H2O | 11.00 | g | ||
|
|
CaCl2 x 2 H2O | 7.34 | g | ||
|
|
MnCl2 x 4 H2O | 2.50 | g | ||
|
|
CoCl2 x 6 H2O | 0.50 | g | ||
|
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(NH4)6Mo7O24 x 4 H2O | 0.50 | g | ||
|
|
FeSO4 x 7 H2O | 5.00 | g | ||
|
|
CuSO4 x 5 H2O | 0.20 | g | ||
|
|
NaOH | 11.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH. | |||||