Plates; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Trypticase soy broth
(BBL 11768, Oxoid CM129 or Merck 5459) |
30 | g | ||
|
Agar | 20 | g | ||
|
Distilled water | 1000 | ml | ||
1 pH 7.3 | |||||
2 Autoclave at 121°C for 15 min. |