535 .
TRYPTICASE SOY BROTH AGAR
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* Medium for Xenorhabdus bovienii DSM 4766
Rehydrate and grow freeze dried cells from ampoules in liquid medium. Subsequent subculturing may be carried out in liquid medium or on agar plates.
Growth conditions: 2-3 days
aerobic
28 °C
Equipment needed:
Autoclave
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase soy broth
(BBL 11768, Oxoid CM129 or Merck 5459) |
30 | g | ||
|
|
Agar | 20 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 pH 7.3 | |||||
| 2 Autoclave at 121°C for 15 min. | |||||