764 .
HALOANAEROBIUM LACUSROSEUS MEDIUM
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Selected strain: Halanaerobium lacusrosei DSM 10165
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Equipment needed:
Autoclave
Hungate tubes
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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NH4Cl | 1.0 | g | ||
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K2HPO4 | 0.3 | g | ||
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KH2PO4 | 0.3 | g | ||
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MgCl2 x 6 H2O | 2.0 | g | ||
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CaCl2 x 2 H2O | 0.2 | g | ||
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KCl | 4.0 | g | ||
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Na-acetate | 1.0 | g | ||
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Glucose | 10.0 | g | ||
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Trypticase | 0.5 | g | ||
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NaCl | 200.0 | g | ||
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Modified Wolin's mineral solution II | 1.0 | ml | ||
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Resazurin | 1.0 | mg | ||
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Yeast extract | 1.0 | g | ||
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Distilled water | 1000.0 | ml | ||
| 1 Prepare the medium and boil, cool under N2:CO2 (80:20 v/v). Dispense medium into Hungate tubes or serum bottles under N2:CO2 (80:20 v/v), autoclave. To the sterile medium add from sterile stock solutions 2% Na2S x 9 H2O (0.1ml/10ml), 10% NaHCO3 (0.5ml/10ml), and 1M glucose (0.25ml/10ml). Final pH 7.0. | |||||
Modified Wolin's mineral solution II #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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Nitrilotriacetic acid | 1.500 | g | ||
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MgSO4 x 7 H2O | 3.000 | g | ||
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MnSO4 x H2O | 0.500 | g | ||
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NaCl | 1.000 | g | ||
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FeSO4 x 7 H2O | 0.100 | g | ||
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CoSO4 x 7 H2O | 0.180 | g | ||
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CaCl2 x 2 H2O | 0.100 | g | ||
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ZnSO4 x 7 H2O | 0.180 | g | ||
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CuSO4 x 5 H2O | 0.010 | g | ||
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AlK(SO4)2 x 12 H2O | 0.020 | g | ||
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H3BO3 | 0.010 | g | ||
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Na2MoO4 x 2 H2O | 0.010 | g | ||
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NiCl2 x 6 H2O | 0.025 | g | ||
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Na2SeO3 x 5 H2O | 0.300 | mg | ||
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Distilled water | 1000.000 | ml | ||
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||