DSMZ 818 . CHRYSIOGENES MEDIUM

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Equipment needed: Autoclave Filter Gassing station Hungate tubes

Final gas composition: 100 % N2 read more

Final pH: 7.4 - 7.8

Select a strain (optional):

ml
Compound Amount Unit
NaCl 1.20 g
KCl 0.30 g
NH4Cl 0.30 g
KH2PO4 0.20 g
Na2SO4 0.30 g
MgCl2 x 6 H2O 0.40 g
CaCl2 x 2 H2O 0.15 g
Trace element solution SL-11 1.00 ml
Sodium resazurin
(0.1% w/v)
0.50 ml
NaHCO3 0.60 g
KNO3 0.50 g
Wolin's vitamin solution (10x) 1.00 ml
Seven vitamins solution 1.00 ml
Na-acetate 0.50 g
Distilled water 1000.00 ml
1 Dissolve ingredients except bicarbonate, nitrate, vitamins and acetate. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, adjust pH to 7.0 - 7.4, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add nitrate, vitamins and acetate from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the complete medium should be adjusted to 7.4 - 7.8, if necessary.

Trace element solution SL-11 #

1000
Compound Amount Unit
Na2-EDTA x 2 H2O 5.2 g
FeCl2 x 4 H2O 1.5 g
ZnCl2 70.0 mg
MnCl2 x 4 H2O 100.0 mg
H3BO3 6.0 mg
CoCl2 x 6 H2O 190.0 mg
CuCl2 x 2 H2O 2.0 mg
NiCl2 x 6 H2O 24.0 mg
Na2MoO4 x 2 H2O 36.0 mg
Distilled water 1000.0 ml
1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.