830 .
R2A MEDIUM
What is this page?
* Medium for Aromatoleum toluvorans DSM 15124
Aerobic. Rehydrate and grow lyophilized cells from ampoules in liquid broth 830. Subsequent subculturing may be carried out in liquid medium or on agar plates.
Growth conditions: 30 °C
Equipment needed:
Autoclave
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 0.50 | g | ||
|
|
Proteose peptone
(Difco no. 3) |
0.50 | g | ||
|
|
Casamino acids | 0.50 | g | ||
|
|
Glucose | 0.50 | g | ||
|
|
Starch
(soluble) |
0.50 | g | ||
|
|
Na-pyruvate | 0.30 | g | ||
|
|
K2HPO4 | 0.30 | g | ||
|
|
MgSO4 x 7 H2O | 0.05 | g | ||
|
|
Agar | 15.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C. | |||||