830 .
R2A MEDIUM
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* Medium for Telmatobacter bradus DSM 23630
(leave out phosphate and pyruvate. Dilute 1:10 and adjust pH to 4.5 - 5.0 with alginic acid (or MES)); fill tubes for anaerobes (Hungate) half with medium, close tightly. Do not shake.
Growth conditions: 22 °C
Equipment needed:
Autoclave
Final pH:
* 4.5 - 5.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 0.50 | g | ||
|
|
Proteose peptone
(Difco no. 3) |
0.50 | g | ||
|
|
Casamino acids | 0.50 | g | ||
|
|
Glucose | 0.50 | g | ||
|
|
Starch
(soluble) |
0.50 | g | ||
| Na-pyruvate* | 0.30 | g | |||
| K2HPO4* | 0.30 | g | |||
|
|
MgSO4 x 7 H2O | 0.05 | g | ||
|
|
Agar | 15.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C. | |||||