904 .
MARINITOGA MEDIUM
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Selected strain: Caloranaerobacter azorensis DSM 13643
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
65 °C
Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
7.0
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Sea Salt
(SIGMA) |
30.0 | g | ||
|
|
PIPES
(SIGMA) |
6.0 | g | ||
|
|
Yeast extract | 1.0 | g | ||
|
|
Tryptone
(BD Bacto) |
1.0 | g | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | ||
|
|
D-Glucose | 2.5 | g | ||
|
|
Na2S x 9 H2O | 0.5 | g | ||
|
|
L-Cysteine HCl x H2O | 0.5 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve ingredients except glucose, sulfide and cysteine, adjust pH to 7.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (to 30% of volume) and autoclave. Add glucose, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. | |||||