951 .
METHYLOPHAGA SULFIDOVORANS MEDIUM
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Selected strain: Methylophaga sulfidovorans DSM 11578
This strain uses the standard medium without modifications.
Growth conditions: 25 °C
Equipment needed:
Filter
Hungate tubes
Final pH:
7.2 - 7.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 15.00 | g | ||
|
|
(NH4)2SO4 | 0.50 | g | ||
|
|
CaCl2 x 6 H2O | 0.33 | g | ||
|
|
KCl | 0.20 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
KH2PO4 | 0.02 | g | ||
|
|
Na2CO3 | 2.00 | g | ||
|
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FeSO4 x 7 H2O | 1.00 | mg | ||
|
|
Trace element solution SL10 | 1.00 | ml | ||
|
|
Vitamin solution | 1.00 | ml | ||
|
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Methanol | 10.00 | ml | ||
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Agar | 15.00 | g | ||
|
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Distilled water | 998.00 | ml | ||
| 1 Prepare the medium without methanol and the vitamin solution. Adjust pH to 7.5 (±0.3) with 2 N HCl. Liquid media is prepared in either Hungate tubes (5ml medium) or 100ml serum bottles (10ml medium) sealed with rubber stoppers. | |||||
| 2 Sterilize by autoclaving. After autoclaving add the methanol (filter sterilised) and the vitamin solution (filter sterilised) from sterile stock solutions. | |||||
Trace element solution SL10 #
1005
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(concentrated) |
5.000 | ml |
|
|
FeCl2 x 4 H2O | 1.500 | g |
|
|
ZnCl2 | 0.070 | g |
|
|
MnCl2 x 4 H2O | 0.200 | g |
|
|
H3BO3 | 6.000 | mg |
|
|
CoCl2 x 6 H2O | 0.190 | g |
|
|
CuCl2 x 2 H2O | 2.000 | mg |
|
|
NiCl2 x 6 H2O | 0.024 | g |
|
|
Na2MoO4 x 2 H2O | 0.036 | g |
|
|
Distilled water | 1000.000 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 20 | mg |
|
|
Nicotinic acid | 200 | mg |
|
|
Thiamine | 100 | mg |
|
|
p-Aminobenzoic acid | 100 | mg |
|
|
Pantothenate | 50 | mg |
|
|
Pyridoxine hydrochloride | 500 | mg |
|
|
Riboflavine | 10 | mg |
|
|
Vitamin B12 | 10 | mg |
|
|
Distilled water | 1000 | ml |