JCM 1032 . SVX8 MEDIUM

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Equipment needed: Autoclave

Final pH: n.d.

Select a strain (optional):

ml
Compound Amount Unit
NaCl 18.000 g
MgCl2 x 6 H2O 4.330 g
NH4Cl 0.330 g
KCl 0.330 g
KH2PO4 0.165 g
CaCl2 x 2 H2O 0.033 g
Trace elements solution 10.000 ml
Distilled water 1000.000 ml
1 Mix components, bring to a boil and cool down under a CO2 (100%) gas stream. While gassing, add the following components to the medium:
Trace vitamins 10.000 ml
NaHCO3 2.000 g
2 Bubble N2 for 3 - 4 min and check pH to be 6.5 - 6.8. Readjust pH to the range if necessary. Autoclave under a CO2 gas atmosphere. Add the following solution to the medium (separately autoclaved under N2):
Maltose
(w/v, 10%)
20.000 ml
KNO3
(1 M)
10.000 ml

Trace elements solution #

1000
Compound Amount Unit
Fe(NH4)2(SO4)2 x 6 H2O 78.40 mg
CoCl2 x 6 H2O 23.80 mg
NiCl2 x 6 H2O 16.60 mg
Na2MoO4 x 2 H2O 2.42 mg
Na2WO4 x 2 H2O 5.49 mg
ZnSO4 x 7 H2O 14.40 mg
CuCl2 x 2 H2O 0.17 mg
Na2SeO4 9.45 mg
H3BO3 0.62 mg
MnCl2 x 4 H2O 19.80 mg
SrSO4 0.92 mg
CrK(SO4)2 x 12 H2O 0.50 mg
AlCl3 x 6 H2O 2.41 mg
BaCl2 2.08 mg
Na2SiO3 x 9 H2O 14.20 mg
KBr 11.90 mg
KI 16.60 mg
Na2SO4 71.00 mg
Distilled water 1000.00 ml