JCM 558 . MAGNETOSPIRILLUM MEDIUM (A)

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Selected strain: Magnetospirillum JCM 12777

This strain uses the standard medium without modifications.

Equipment needed: Autoclave Filter

Final pH: 7.3

Select a strain (optional):

ml
Compound Amount Unit
Na2HPO4 1.60 g
KH2PO4 1.00 g
NH4Cl 0.50 g
K2SO4 0.06 g
KNO3 0.51 g
Trace x 10 solution 0.10 ml
Se/W solution 1.00 ml
Mg/Ca solution 5.00 ml
Na benzoate solution 72.0 g/L 2.00 ml
7 Vitamins solution 0.50 ml
FeSO4 solution (5 g/L) with EDTA 3.00 ml
Distilled water 1000.00 ml
1 Adjust pH to 7.2 - 7.4.
2 Separately autoclave Trace x 10 solution, Se/W solution and Mg/Ca solution, and filter-sterilize Na benzoate solution, 7 vitamins solution and FeSO4 solution. Aseptically add all solutions to the medium.

Trace x 10 solution #

100
Compound Amount Unit
H3BO3 6 mg
CuCl2 x 2 H2O 2 mg
Distilled water 100 ml

Se/W solution #

100
Compound Amount Unit
NaOH 0.05 g
Na2SeO3 0.20 mg
Na2WO4 x 2 H2O 0.40 mg
Distilled water 100.00 ml

Mg/Ca solution #

100
Compound Amount Unit
MgCl2 x 6 H2O 2.0 g
CaCl2 x 2 H2O 0.5 g
Distilled water 100.0 ml

Na benzoate solution 72.0 g/L #

100
Compound Amount Unit
Sodium benzoate 7.2 g
Distilled water 100.0 ml

FeSO4 solution (5 g/L) with EDTA #

100
Compound Amount Unit
Na2-EDTA 0.50 g
FeSO4 x 7 H2O 0.05 g
Distilled water 100.00 ml