558 .
MAGNETOSPIRILLUM MEDIUM (A)
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Selected strain: Magnetospirillum JCM 12778
This strain uses the standard medium without modifications.
Equipment needed:
Autoclave
Filter
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2HPO4 | 1.60 | g | ||
|
|
KH2PO4 | 1.00 | g | ||
|
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NH4Cl | 0.50 | g | ||
|
|
K2SO4 | 0.06 | g | ||
|
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KNO3 | 0.51 | g | ||
|
|
Trace x 10 solution | 0.10 | ml | ||
|
|
Se/W solution | 1.00 | ml | ||
|
|
Mg/Ca solution | 5.00 | ml | ||
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Na benzoate solution 72.0 g/L | 2.00 | ml | ||
|
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7 Vitamins solution | 0.50 | ml | ||
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FeSO4 solution (5 g/L) with EDTA | 3.00 | ml | ||
|
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Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.2 - 7.4. | |||||
| 2 Separately autoclave Trace x 10 solution, Se/W solution and Mg/Ca solution, and filter-sterilize Na benzoate solution, 7 vitamins solution and FeSO4 solution. Aseptically add all solutions to the medium. | |||||
Se/W solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.05 | g |
|
|
Na2SeO3 | 0.20 | mg |
|
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Na2WO4 x 2 H2O | 0.40 | mg |
|
|
Distilled water | 100.00 | ml |
Mg/Ca solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
MgCl2 x 6 H2O | 2.0 | g |
|
|
CaCl2 x 2 H2O | 0.5 | g |
|
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Distilled water | 100.0 | ml |
Na benzoate solution 72.0 g/L #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Sodium benzoate | 7.2 | g |
|
|
Distilled water | 100.0 | ml |
7 Vitamins solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Vitamin B12 | 4 | mg |
|
|
D-Biotin | 2 | mg |
|
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D-Calcium pantothenate | 5 | mg |
|
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Thiamine HCl | 10 | mg |
|
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p-Aminobenzoic acid | 10 | mg |
|
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Nicotinic acid | 20 | mg |
|
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Pyridoxine hydrochloride | 50 | mg |
|
|
Distilled water | 100 | ml |
FeSO4 solution (5 g/L) with EDTA #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na2-EDTA | 0.50 | g |
|
|
FeSO4 x 7 H2O | 0.05 | g |
|
|
Distilled water | 100.00 | ml |