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MH1 MEDIUM FOR SALIARCHAEUM RUBRODURUM
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Selected strain: Halarchaeum rubridurum DSM 22443
This strain uses the standard medium without modifications.
Equipment needed:
Final pH:
4.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
L-Glutamic acid | 2 | g | ||
|
|
Trisodium citrate x 2 H2O | 2 | g | ||
|
|
KH2PO4 | 1 | g | ||
|
|
NH4Cl | 1 | g | ||
|
|
K2SO4 | 5 | g | ||
|
|
MgCl2 x 6 H2O | 20 | g | ||
|
|
NaCl | 200 | g | ||
|
|
Casamino acids
(BD-Difco) |
4 | g | ||
|
|
Yeast extract
(BD-Difco) |
2 | g | ||
|
|
FeSO4 x 6 H2O
(0.2%) |
2 | ml | ||
|
|
Trace metal solution | 2 | ml | ||
| 1 For preparation of solid medium, add the components listed above (reduce the amount of NaCl to 150.0 g) to distilled water and bring volume to 800.0 ml. Separately prepare the following solution: | |||||
|
|
NaCl | 50 | g | ||
|
|
Agar | 20 | g | ||
|
|
Distilled water | 200 | ml | ||
| 2 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 5.0 with KOH. | |||||
| 3 Combine both solutions after autoclaving. | |||||
Trace metal solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
CoCl2 x 6 H2O | 0.30 | g | ||
|
|
MnCl2 x 4 H2O | 0.10 | g | ||
|
|
ZnCl2 | 0.10 | g | ||
|
|
H3BO4 | 0.02 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.10 | g | ||
|
|
NiCl2 x 6 H2O | 0.10 | g | ||
|
|
CuCl2 x 2 H2O | 0.01 | g | ||
|
|
AlCl3 | 0.04 | g | ||
|
|
Na2WO4 x 2 H2O | 0.02 | g | ||
|
|
CaCl2 x 2 H2O | 1.00 | g | ||
|
|
BaCl2 x 2 H2O | 0.10 | g | ||
|
|
Na2S2O3 x 5 H2O | 2.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 4.0 with HCl. | |||||