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DSMZ

Strain modifications for Medium 1011

4 distinct modifications were found.

Omit pressurizing vials with 80% N2 and 20% CO2 gas mixture.

Modified strains

Hydrogenovibrio crunogenus DSM 28671

Omit thiosulfate and supplement medium with 4.00 g/l yeast extract and 4.00 g/l Trypton peptone. After autoclaving the medium is reduced with 0.30 g/l Na2S x 9 H2O added from a sterile anoxic stock solution (3% w/v) prepared under 100% N2 gas and the pH adjusted to 6.5. After inoculation pressurize vessels to 1 bar overpressure with sterile 80% N2 and 20% CO2 gas mixture. Do not add sterile air!

Modified strains

Thermotomaculum hydrothermale DSM 24660

Annotated modifications

80% N2, 20% CO2
Na2S2O3 x 5 H2O
6.5
4 g Yeast extract
0.3 g Na2S x 9 H2O
4 g Tryptone peptone

Supplement medium with 2.00 g/l KNO3. Do not add overpressure of 80% N2 and 20% CO2 and do not add sterile air!

Modified strains

Sulfurovum riftiae DSM 101780
Varunaivibrio sulfuroxidans DSM 101688

Annotated modifications

2 g KNO3
80% N2, 20% CO2

Supplement medium with 2.00 g/l NaNO3. After inoculation pressurize vessels to 1 bar overpressure with sterile 80% N2 and 20% CO2 gas mixture. Do not add sterile air!

Modified strains

Sulfurovum lithotrophicum DSM 23290

Annotated modifications

80% N2, 20% CO2
2 g NaNO3
Modifications
Omit pressurizing vials with 80% N2 and 20% CO2 gas mixture. Omit thiosulfate and supplement medium with 4.00 g/l yeast extract and 4.00 g/l Trypton peptone. After autoclaving the medium is reduced with 0.30 g/l Na2S x 9 H2O added from a sterile anoxic stock solution (3% w/v) prepared under 100% N2 gas and the pH adjusted to 6.5. After inoculation pressurize vessels to 1 bar overpressure with sterile 80% N2 and 20% CO2 gas mixture. Do not add sterile air! Supplement medium with 2.00 g/l KNO3. Do not add overpressure of 80% N2 and 20% CO2 and do not add sterile air! Supplement medium with 2.00 g/l NaNO3. After inoculation pressurize vessels to 1 bar overpressure with sterile 80% N2 and 20% CO2 gas mixture. Do not add sterile air!