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Strain modifications for Medium 1011c

3 distinct modifications were found..

Supplement medium with 1.00 g/l NaNO3 and omit pyruvate, lactate and yeast extract. Upon autoclaving add 3.00 g/l sterile sulfur powder (sterilized by steaming for 3 hours on each of 3 successive days) and adjust pH to 6.0 - 6.5.

Modified strains

Thiofractor thiocaminus DSM 22050

Annotated modifications

1.00 g/l NaNO3
3.00 g/l sulfur
Na-pyruvate
Yeast extract
Na lactate
6.0 - 6.5

Supplement medium with 1.00 g/l NaNO3 and omit pyruvate, lactate, yeast extract and dithionite. Upon autoclaving add 10.00 g/l sterile sulfur powder (sterilized by steaming for 3 hours on each of 3 successive days) and adjust pH to 6.0 - 6.5. After inoculation do not pressurize with 80% H2 and 20% CO2 gas mixture and add sterile air in an amount that is equivalent to a volume of 10% of the headspace.

Modified strains

Sulfurovum aggregans DSM 27205

Annotated modifications

1.00 g/l NaNO3
10.00 g/l sulfur
80% H2, 20% CO2
Na-pyruvate
Yeast extract
Na lactate
Sodium dithionite
6.0 - 6.5

Anaerobic

Modified strains

Desulfothermus okinawensis DSM 17375