Strain modifications for Medium 1011c
3 distinct modifications were found.
Omit Na-dithionite solution (5% w/v), Yeast extract, Na2S2O3 x 5 H2O, Na-pyruvate, and Na-lactate. Incubate with a gas phase of 75% H2, 15% CO2, and 10% sterile air. Reduce overpressure to 0.5 bar.
Yeast extract | |
Na-pyruvate | |
Na-lactate | |
Na2S2O3 x 5 H2O | |
- | Na-dithionite solution (5% w/v) |
75% H2, 15% CO2, and 10% sterile air. Reduce overpressure to 0.5 bar |
Supplement medium with 1.00 g/l NaNO3 and omit pyruvate, lactate and yeast extract. Upon autoclaving add 3.00 g/l sterile sulfur powder (sterilized by steaming for 3 hours on each of 3 successive days) and adjust pH to 6.0 - 6.5.
Supplement medium with 1.00 g/l NaNO3 and omit pyruvate, lactate, yeast extract and dithionite. Upon autoclaving add 10.00 g/l sterile sulfur powder (sterilized by steaming for 3 hours on each of 3 successive days) and adjust pH to 6.0 - 6.5. After inoculation do not pressurize with 80% H2 and 20% CO2 gas mixture and add sterile air in an amount that is equivalent to a volume of 10% of the headspace.