2 distinct modifications were found.
For 10 ml in hungate tube, add: Vitamin solution 5-10 µl, Rumen fluid 500 µl, and Cellobiose (10%) 200 µl
For 10 ml in hungate tube, add: Vitamin solution 5-10 µl, Rumen fluid 500 µl, Cellobiose (10%) 200 µl, CM-Mix 500 µl and Vitamin K1 solution 5-10 µl