MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

Strain modifications for Medium 215

38 distinct modifications were found.

Adjust pH of final medium to 9.0.

Modified strains

Nesterenkonia sp. DSM 15840

Annotated modifications

9.0

(this medium is recommended for the routine cultivation of the strain)

Modified strains

Arsenophonus nasoniae DSM 15247

1% arginine

Modified strains

Eggerthella lenta DSM 110911
Eggerthella lenta DSM 110912

Annotated modifications

10 g/l Arginine

add 0,002 ml/ml Phenosafranin (Stock solution 1),add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).

Modified strains

Adlercreutzia muris DSM 110078

Annotated modifications

0.05 g/ml Cysteine
0.02 g/ml DTT

add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).

Modified strains

Bacteroides uniformis DSM 110080
Bacteroides xylanisolvens DSM 110079
Phocaeicola sartorii DSM 110081
Lactococcus lactis DSM 110082
Veillonella intestinalis DSM 110113
Flintibacter muris DSM 110149
Streptococcus caecimuris DSM 110150
Acetatifactor aquisgranensis DSM 110981
Otoolea muris DSM 111138
Intestinimonas aquisgranensis DSM 112680
Hominisplanchenecus murintestinalis DSM 111139
Intestinicryptomonas porci DSM 114844

Annotated modifications

0.02 g/ml DTT
0.05 g/ml Cysteine

Add 5 µg/ml chloramphenicol

Modified strains

Enterococcus faecalis DSM 8629

Annotated modifications

5 mg/l Chloramphenicol

add mouse serum (0,5 ml/10 ml)

Modified strains

Adlercreutzia murintestinalis DSM 112345

Advice for culturing: Please take liquid medium for the first step of cultivation

Modified strains

Actinobacillus succinogenes DSM 22257

Aerobic or anaerobic under N2, plus 2 g/l KNO3; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 215. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Thauera sp. DSM 14975

Annotated modifications

2.0 g/l KNO3

Aerobic or anaerobic with 2 g/l KNO3 under N2-gas; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 215. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Thauera sp. DSM 14973

Annotated modifications

2.0 g/l KNO3

Aerobic. Agar surface must be moist. Best growth results in shaken liquid medium

Modified strains

Thauera terpenica DSM 12140

Anaerobic, N2CO2 plus H2 (1 bar)

Modified strains

Peptoniphilus porci DSM 104947

Anaerobic, N2Co2 plus H2

Modified strains

Intestinimonas butyriciproducens DSM 104946

Anaerobic, N2CO2 plus H2 (1 bar)

Modified strains

Suipraeoptans intestinalis DSM 104945
Hornefia porci DSM 104948

Cultivate rehydrated cells in liquid medium first. Subsequent cultivation possible in liquid broth and on agar plates.

Modified strains

Iodobacter fluviatilis DSM 3764

Does not grow reliably on agar plates

Modified strains

Snodgrassella alvi DSM 104735

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).

Modified strains

needs high humidity

Modified strains

Neisseria wadsworthii DSM 22245
Neisseria wadsworthii DSM 22247
Neisseria shayeganii DSM 22246
Neisseria shayeganii DSM 22244

Plus 1% arginine

Modified strains

Ructibacterium gallinarum DSM 107454
Claveliimonas monacensis DSM 108991
Eggerthella lenta DSM 110904
Eggerthella lenta DSM 110905
Eggerthella lenta DSM 110906
Eggerthella lenta DSM 110910
Eggerthella lenta DSM 110914
Eggerthella lenta DSM 110915
Eggerthella lenta DSM 110917
Eggerthella lenta DSM 110919
Eggerthella lenta DSM 110920
Eggerthella lenta DSM 110921
Eggerthella lenta DSM 110922
Eggerthella lenta DSM 110923
Gordonibacter pamelaeae DSM 110924
Gordonibacter sp. DSM 110925

Annotated modifications

10.0 g/l Arginine

Plus 10 mg MnSO4

Modified strains

Alkalihalobacillus okuhidensis DSM 13667

Annotated modifications

10.0 mg MnSO4

Plus 10 mg MnSO4, pH 10.0

Modified strains

Alkalihalobacillus okuhidensis DSM 13666

Annotated modifications

10.0 mg MnSO4
10.0

Plus 10 µg/ml MnSO4

Modified strains

Sporosarcina luteola DSM 23150

Annotated modifications

10 µg/ml MnSO4

Plus 10% bovine serum plus 1 % maltose plus haemin

Modified strains

Gardnerella vaginalis DSM 4944

Annotated modifications

10.0 g/l Maltose
10 % Bovine serum

Plus 15% rumen fluid; strictly anaerobic

Modified strains

Actinomyces ruminicola DSM 27982

Annotated modifications

150.0 g/l Rumen fluid

Plus 18 g/l NaCl

Modified strains

Photobacterium damselae subsp. piscicida DSM 22834

Annotated modifications

18.0 g/l NaCl

Plus 2 g/l KNO3, anaerobic under N2

Modified strains

Thauera sp. DSM 14974

Annotated modifications

2.0 g/l KNO3

Plus 2g/l KNO3, anaerobic under N2, or 215 aerobic

Modified strains

Thauera sp. DSM 14976

Annotated modifications

2.0 g/l KNO3

Plus 5 % rabbit blood

Modified strains

Bartonella rochalimae DSM 25050

Annotated modifications

50.0 g/l Rabbit blood

Plus IsovitaleX (5 ml / liter)

Modified strains

Actinobacillus pleuropneumoniae DSM 21511

Annotated modifications

5 ml/l Isovitalex

Plus 0.72 g/l KNO3; anaerobic (N2)

Modified strains

Comamonas nitrativorans DSM 13191

Annotated modifications

0.72 g/l KNO3

Plus 1% arginine

Modified strains

Eggerthella lenta DSM 110907
Eggerthella lenta DSM 110908
Eggerthella lenta DSM 110909
Eggerthella lenta DSM 110913
Eggerthella lenta DSM 110916
Eggerthella lenta DSM 110918
Paraeggerthella hongkongensis DSM 110927
Eggerthella lenta DSM 110928

Annotated modifications

10.0 g/l Arginine

plus 1% malttose plus 10% FBS

Modified strains

Gardnerella vaginalis DSM 104275
Gardnerella vaginalis DSM 104282
Gardnerella vaginalis DSM 104283
Gardnerella vaginalis DSM 104284

plus horse serum (5% vol/vol)

Modified strains

Erysipelothrix urinaevulpis DSM 106013

plus maltose (10 g/l) plus bovine serum (10 %)

Modified strains

Gardnerella vaginalis DSM 104281

plus Tween 80

Modified strains

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1 or 215. Subsequent subculturing may be carried out in liquid medium or on agar plates.

Modified strains

Cupriavidus necator DSM 13513

Supplemented with soil extract (50%)

Modified strains

Heyndrickxia sporothermodurans DSM 10599

Annotated modifications

50 % Soil extract

Adjust pH of final medium to 9.5 - 10.0.

Modified strains

Streptomyces sannurensis DSM 41834

Annotated modifications

9.5 - 10.0
Modifications
Adjust pH of final medium to 9.0. (this medium is recommended for the routine cultivation of the strain) 1% arginine add 0,002 ml/ml Phenosafranin (Stock solution 1),add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). Add 5 µg/ml chloramphenicol add mouse serum (0,5 ml/10 ml) Advice for culturing: Please take liquid medium for the first step of cultivation Aerobic or anaerobic under N2, plus 2 g/l KNO3; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 215. Subsequent subculturing may be carried out in liquid medium or on agar plates Aerobic or anaerobic with 2 g/l KNO3 under N2-gas; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 215. Subsequent subculturing may be carried out in liquid medium or on agar plates Aerobic. Agar surface must be moist. Best growth results in shaken liquid medium Anaerobic, N2CO2 plus H2 (1 bar) Anaerobic, N2Co2 plus H2 Anaerobic, N2CO2 plus H2 (1 bar) Cultivate rehydrated cells in liquid medium first. Subsequent cultivation possible in liquid broth and on agar plates. Does not grow reliably on agar plates Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). needs high humidity Plus 1% arginine Plus 10 mg MnSO4 Plus 10 mg MnSO4, pH 10.0 Plus 10 µg/ml MnSO4 Plus 10% bovine serum plus 1 % maltose plus haemin Plus 15% rumen fluid; strictly anaerobic Plus 18 g/l NaCl Plus 2 g/l KNO3, anaerobic under N2 Plus 2g/l KNO3, anaerobic under N2, or 215 aerobic Plus 5 % rabbit blood Plus IsovitaleX (5 ml / liter) Plus 0.72 g/l KNO3; anaerobic (N2) Plus 1% arginine plus 1% malttose plus 10% FBS plus horse serum (5% vol/vol) plus maltose (10 g/l) plus bovine serum (10 %) plus Tween 80 Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1 or 215. Subsequent subculturing may be carried out in liquid medium or on agar plates. Supplemented with soil extract (50%) Adjust pH of final medium to 9.5 - 10.0.