Strain modifications for Medium 339a
6 distinct modifications were found.
Adjust pH of medium after autoclaving to 7.4 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere.
Adjust pH of medium to 6.0.
Omit D-glucose.
Replace D-glucose with 3.2 g/l Na2-fumarate prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.2.
Replace D-glucose with 5.0 g/l cellobiose prepared under 100% N2 gas atmosphere and sterilized by filtration.