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DSMZ

Strain modifications for Medium 383

18 distinct modifications were found.

Change amount of pyruvate to 2.20 g/l and add 0.10 g/l yeast extract.

Modified strains

Dethiosulfatarculus sandiegensis DSM 100305
Petrothermobacter organivorans DSM 105015

Annotated modifications

Na-pyruvate
0.1 g Yeast extract
to Solution C
2.20 g Na-pyruvate
to Solution C

Naphthalene dissolved in heptamethylnonane can be used as substrate instead of pyruvate.

Modified strains

unclassified bacterium DSM 14454

Annotated modifications

0.4 g Naphthalene
to Solution C
20.00 ml 2,2,4,4,6,8,8-Heptamethylnonane
to Solution C
Distilled water
to Solution C
Na-pyruvate
to Solution C

Replace pyruvate with 0.30 g/l p-hydroxybenzoate.

Modified strains

Desulfobacterium sp. DSM 8540

Annotated modifications

Na-pyruvate
0.3 g p-Hydroxybenzoate
to Solution C

Replace pyruvate with 0.50 g/l yeast extract and 0.68 g/l sodium formate.

Modified strains

Desulfoplanes formicivorans DSM 28890

Annotated modifications

Na-pyruvate
0.5 g Yeast extract
to Solution C
0.68 g Sodium formate
to Solution C

Replace pyruvate with 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate, and 0.15 g/l sodium octanoate.

Modified strains

Desulfocella sp. DSM 2056

Annotated modifications

Na-pyruvate
0.7 g Na-butyrate
to Solution C
0.3 g Na-caproate
to Solution C
0.15 g Sodium octanoate
to Solution C

Replace pyruvate with 0.90 ml/l 1-butanol.

Modified strains

Desulfoconvexum algidum DSM 21856

Annotated modifications

Na-pyruvate
0.9 ml 1-Butanol
to Solution C

Replace pyruvate with 1.00 g/l valeric acid.

Modified strains

Desulfatibacillum sp. DSM 12861
Desulfatitalea sp. DSM 12883

Annotated modifications

Na-pyruvate
1.0 g Valeric acid
to Solution C

Replace pyruvate with 1.00 g/l betaine.

Modified strains

Desulfobacterium sp. DSM 13036

Annotated modifications

Na-pyruvate
1.0 g Betaine
to Solution C

Replace pyruvate with 1.00 g/l Na-caprylate and supplement medium with 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration.

Modified strains

Desulfatibacillum aliphaticivorans DSM 15576
Desulfatibacillum alkenivorans DSM 16219

Annotated modifications

+ 1.0 ml Seven vitamins solution
Na-pyruvate
1.0 g Na-caprylate
to Solution C

Replace pyruvate with 1.00 g/l Na-glycolate sterilized by filtration.

Modified strains

Desulfofustis glycolicus DSM 9705

Annotated modifications

Na-pyruvate
1 g Na-glycolate
to Solution C

Replace pyruvate with 1.10 g/l Na-butyrate and 1.10 g/l caproic acid.

Modified strains

Desulfotignum balticum DSM 12888

Annotated modifications

Na-pyruvate
1.1 g Na-butyrate
to Solution C
1.1 g Caproic acid
to Solution C

Replace pyruvate with 1.90 g/l sodium glutamate monohydrate and 1.00 g/l yeast extract.

Modified strains

Dethiosulfatibacter aminovorans DSM 17477

Annotated modifications

Na-pyruvate
1.9 g Sodium glutamate monohydrate
to Solution C
1.0 g Yeast extract
to Solution C

Replace pyruvate with 2.00 g/l malonic acid.

Modified strains

Malonomonas rubra DSM 5091
Malonomonas rubra DSM 9788

Annotated modifications

Na-pyruvate
2.0 g Malonic acid
to Solution C

Replace pyruvate with 3.00 g/l Casamino acids.

Modified strains

Thermovirga lienii DSM 17291

Annotated modifications

Na-pyruvate
3.0 g Casamino acids
to Solution C

Replace pyruvate with indole as substrate. Dissolve 0.30 g of indole in 90 ml water by heating and shaking in a closed bottle under 100% N2 gas atmosphere, autoclave, then add 7.00 ml of a sterile anoxic stock solution of NaCl (30% w/v) and 0.70 ml of a sterile anoxic stock solution of MgCl2 x 6 H2O (40% w/v). Store the indole-salt solution in the dark. Reheat and shake before use. Add to sterile medium 30.00 ml/l of the indole-salt solution in the beginning, and 2 x 30.00 ml/l during growth.

Modified strains

Desulfobacterium indolicum DSM 3383

Annotated modifications

- Solution C
+ 90.00 ml Indole solution (0.3 %)

Replace pyurvate with resorcinol. During growth the culture is fed once with the same amount of resorcinol.

Modified strains

unclassified bacterium DSM 4661

Annotated modifications

Na-pyruvate
0.11 g Resorcinol
to Solution C

Supplement medium with 1.00 ml/l seven vitamins solution (see medium 503). Replace pyruvate with 0.40 g/l benzoate and 0.10 g/l yeast extract sterilized separately by filtration and add to the autoclaved medium from anoxic stock solutions.

Modified strains

Desulfotignum balticum DSM 7044
Desulfobacterium sp. DSM 7120
Desulfobacula toluolica DSM 7467
unclassified bacterium DSM 12567
Desulfosarcina ovata DSM 13228
Desulfosarcina ovata subsp. sediminis DSM 23484

Annotated modifications

+ 1.0 ml Seven vitamins solution
Na-pyruvate
0.4 g Na-benzoate
to Solution C
0.1 g Yeast extract
to Solution C

The vitamin solution of medium 120 is replaced with 1.00 ml/l of seven vitamins solution of medium 503. Replace pyruvate with 0.40 g/l Na-benzoate and add 10.00 ml/l clarified rumen fluid (see medium 1310) as growth supplement. Initiation of growth requires addition of freshly prepared sodium dithionite.

Modified strains

Desulfobacula phenolica DSM 3384

Annotated modifications

+ 0.5 ml Na-dithionite solution (5% w/v)
- Wolin's vitamin solution (10x)
+ 10.0 ml Clarified rumen fluid
Na-pyruvate
0.4 g Na-benzoate
to Solution C
+ 1.0 ml Seven vitamins solution
to Solution D
Modifications
Change amount of pyruvate to 2.20 g/l and add 0.10 g/l yeast extract. Naphthalene dissolved in heptamethylnonane can be used as substrate instead of pyruvate. Replace pyruvate with 0.30 g/l p-hydroxybenzoate. Replace pyruvate with 0.50 g/l yeast extract and 0.68 g/l sodium formate. Replace pyruvate with 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate, and 0.15 g/l sodium octanoate. Replace pyruvate with 0.90 ml/l 1-butanol. Replace pyruvate with 1.00 g/l valeric acid. Replace pyruvate with 1.00 g/l betaine. Replace pyruvate with 1.00 g/l Na-caprylate and supplement medium with 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Replace pyruvate with 1.00 g/l Na-glycolate sterilized by filtration. Replace pyruvate with 1.10 g/l Na-butyrate and 1.10 g/l caproic acid. Replace pyruvate with 1.90 g/l sodium glutamate monohydrate and 1.00 g/l yeast extract. Replace pyruvate with 2.00 g/l malonic acid. Replace pyruvate with 3.00 g/l Casamino acids. Replace pyruvate with indole as substrate. Dissolve 0.30 g of indole in 90 ml water by heating and shaking in a closed bottle under 100% N2 gas atmosphere, autoclave, then add 7.00 ml of a sterile anoxic stock solution of NaCl (30% w/v) and 0.70 ml of a sterile anoxic stock solution of MgCl2 x 6 H2O (40% w/v). Store the indole-salt solution in the dark. Reheat and shake before use. Add to sterile medium 30.00 ml/l of the indole-salt solution in the beginning, and 2 x 30.00 ml/l during growth. Replace pyurvate with resorcinol. During growth the culture is fed once with the same amount of resorcinol. Supplement medium with 1.00 ml/l seven vitamins solution (see medium 503). Replace pyruvate with 0.40 g/l benzoate and 0.10 g/l yeast extract sterilized separately by filtration and add to the autoclaved medium from anoxic stock solutions. The vitamin solution of medium 120 is replaced with 1.00 ml/l of seven vitamins solution of medium 503. Replace pyruvate with 0.40 g/l Na-benzoate and add 10.00 ml/l clarified rumen fluid (see medium 1310) as growth supplement. Initiation of growth requires addition of freshly prepared sodium dithionite.