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DSMZ

Strain modifications for Medium 383b

3 distinct modifications were found.

Adjust pH of final medium to 6.6.

Modified strains

Desulfolithobacter dissulfuricans DSM 111414

Annotated modifications

6.6

Supplement medium with 1.00 g/l Na-acetate and 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from sterile and anoxic stock solutions. Vitamins are sterilized by filtration. Adjust pH of medium to 6.5 - 6.7 prior to inoculation.

Modified strains

Thermodesulfatator indicus DSM 15286
Thermodesulfatator atlanticus DSM 21156
Thermodesulfatator autotrophicus DSM 101864

Annotated modifications

6.5 - 6.7
+ 1 ml Seven vitamins solution
1.00 g Na-acetate
to Solution A

Supplement medium with 1.00 g/l Na-acetate, 2.00 g/l Trypticase peptone and 2.00 g/l yeast extract added to the autoclaved medium from sterile anoxic stock solutions prepared under N2 gas.

Modified strains

Desulfonauticus submarinus DSM 15269
Desulfonauticus autotrophicus DSM 4206

Annotated modifications

2 g Yeast extract
to Solution A
2 g Trypticase peptone
to Solution A
1.00 g Na-acetate
to Solution A
Modifications
Adjust pH of final medium to 6.6. Supplement medium with 1.00 g/l Na-acetate and 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from sterile and anoxic stock solutions. Vitamins are sterilized by filtration. Adjust pH of medium to 6.5 - 6.7 prior to inoculation. Supplement medium with 1.00 g/l Na-acetate, 2.00 g/l Trypticase peptone and 2.00 g/l yeast extract added to the autoclaved medium from sterile anoxic stock solutions prepared under N2 gas.