Strain modifications for Medium 457

125 distinct modifications were found.

(add benzene to the incubation atmosphere)

Modified strains

Pseudomonas stutzeri DSM 8219
Pseudomonas benzenivorans DSM 8628

(add camphor to the incubation atmosphere)

Modified strains

Pseudomonas sp. DSM 7162

(add dibenzofurane to the incubation atmosphere)

Modified strains

Sphingomonas sp. DSM 7135

(add first benzene then 1,4-dichlorobenzene to the incubation atmosphere)

Modified strains

Paraburkholderia sp. DSM 6818

(add isopropanol to the incubation atmosphere)

Modified strains

Rhodococcus ruber DSM 8425

(add methoxy-2-propanol to the incubation atmosphere)

Modified strains

Rhodococcus erythropolis DSM 8424

(add naphthalene to the incubation atmosphere)

Modified strains

Cupriavidus basilensis DSM 9750

(add o-xylene to the incubation atmosphere)

Modified strains

Rhodococcus opacus DSM 8531

(add toluene to the incubation atmosphere)

Modified strains

Pseudomonas putida DSM 3226

(after autoclaving add 10 ml/l of a neutralized filter-sterilized solution of 5 g/l 4-nitrophenol); Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 220. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Pseudomonas sp. DSM 6837

(after autoclaving add 10 mmol/l dichloromethane and/or methanol)

Modified strains

Methylorubrum extorquens DSM 6343

Annotated modifications

10 mM Dichloromethane

(after autoclaving and cooling, add 1 g/l filter-sterilized trans-caryophyllene)

Modified strains

Gordonia sp. DSM 6391

Annotated modifications

1 g/l (-)-Caryophyllene

(after autoclaving and cooling, add 1 ml/l filter-sterilized trans-caryophyllene)

Modified strains

Gordonia sp. DSM 6377

Annotated modifications

1 ml/l (-)-Caryophyllene

(after autoclaving, add 0.5 g/l aniline from a filter-sterilized stock solution)

Modified strains

Rhodococcus rhodochrous DSM 6263
Rhodococcus ruber DSM 6264

Annotated modifications

0.5 g/l Aniline

(after autoclaving, add 1 g/l chlorobenzoate from a filter-sterilized 10-fold concentrated stock solution)

Modified strains

Pseudomonas aeruginosa DSM 6279

(pH 6, add 0,5 g/l of p-chloroaniline from a filter sterilized stock solution to the autoclaved medium)

Modified strains

Cupriavidus sp. DSM 7346

Annotated modifications

6.0
0.5 g/l 4-Chloroaniline

(pH 7.5, with 100 mg/l yeast extract. After autoclaving add 20 ml of a 1M dichloropropionate filter-sterilized stock solution)

Modified strains

Cupriavidus sp. DSM 6758

Annotated modifications

100.0 mg/l Yeast extract
7.5

(supply 3-chlorotoluene via incubation atmosphere)

Modified strains

(supply 4-chloroaniline via incubation atmosphere)

Modified strains

Achromobacter denitrificans DSM 11850

(supply isopropylbenzene (cumol) via the gas phase)

Modified strains

Pseudomonas fluorescens DSM 6607
Pseudomonas fluorescens DSM 6609

(supply naphthalene via gasphase)

Modified strains

Rhodococcus ruber DSM 7511

(supply o-xylene via incubation atmosphere)

Modified strains

Pseudomonas sp. DSM 6538

(supply p-cresol via the gas phase)

Modified strains

Pseudomonas putida DSM 6521

(supply pseudocumene via incubation atmosphere)

Modified strains

Pseudomonas fluorescens DSM 7122

(supply toluene via the incubation atmosphere)

Modified strains

Rhodococcus rhodochrous DSM 11097

(with 50 mg/l yeast extract, add 1,4-dichlorobenzene to the incubation atmosphere)

Modified strains

Paraburkholderia sp. DSM 7116
Paraburkholderia sp. DSM 8530

Annotated modifications

50.0 mg/l Yeast extract

add 0.2 g/l p-cresol

Modified strains

Pseudomonas fluorescens DSM 7355

Annotated modifications

0.2 g/l Cresol

add 1 g/l of chloro- or fluoroacetate from a neutralized and filter sterilized stock solution to the autoclaved medium

Modified strains

Variovorax sp. DSM 7516

Annotated modifications

1 g/l Sodium chloroacetate

add 1 mmol/l of 4-carboxybiphenyl ether from a filter sterilized stock solution to the autoclaved medium

Modified strains

add 1,2,4-trichlorobenzene to the gas phase

Modified strains

Cupriavidus basilensis DSM 8910

add 1,4-dichlorobenzene to the incubation atmosphere

Modified strains

Xanthobacter flavus DSM 10330

add 100 mg/L cetyltrimethylammonium chloride from a filter sterilized stock solution to the autoclaved medium

Modified strains

Pseudomonas saponiphila DSM 9751

Annotated modifications

100 mg/l Cetyltrimethylammonium

add 2,5 mmol/l 4-sulfophthalate from a filter sterilized stock solution to the autoclaved medium

Modified strains

Comamonas testosteroni DSM 7300

Annotated modifications

2.5 mM 4-sulfophthalate

add 2-chloropropenol to the incubation atmosphere

Modified strains

Pseudomonas fluorescens DSM 8542
Pseudomonas fluorescens DSM 8543
Pseudomonas sp. DSM 8544

add 300 mg/l 1-chloro-4-nitrobenzene to the autoclaved and cooled medium

Modified strains

Diaphorobacter sp. DSM 13225

Annotated modifications

300 mg/l 1-Chloro-4-nitrobenzene

add 4 mM aniline from a filter sterilized stock solution to the autoclaved medium

Modified strains

Delftia tsuruhatensis DSM 8370

Annotated modifications

4 mM Aniline

add 40 ml 4-chlorobenzenesulfonic acid from a 100 mmol/l filter-sterilized stock solution to 1 l autoclaved medium 457

Modified strains

Pseudomonas aeruginosa DSM 8924

Annotated modifications

40 ml 4-chlorobenzenesulfonic

add 5 mmol/l acetanilide from a filter sterilized stock solution (30 mmol/l)

Modified strains

Acinetobacter sp. DSM 7324

Annotated modifications

5 mM Acetanilide

add 5 mmol/l acetanilide from a filter sterilized stock solution (30 mmol/l) to the autoclaved medium

Modified strains

Arthrobacter sp. DSM 7325

Annotated modifications

5 mM Acetanilide

add 50 mg/l of yeast extract and some crystals of 3-chlorodibenzofuran. Transformation is indicated by deep yellow colour of medium.

Modified strains

Pseudomonas moorei DSM 7390

Annotated modifications

50 mg/l Yeast extract

add 50 mg/l yeast extract and 1 g/l fluoroacetate from a filter sterilized stock solution to the autoclaved medium

Modified strains

Caballeronia sp. DSM 8341

Annotated modifications

50.0 mg/l Yeast extract
1 g/l Fluoroacetate

add 640 mg/l dichlorprop [2-(2,4-dichlorophenoxy)propionic acid] from a filtersterilized stock solution to the sterilized and cooled medium

Modified strains

Sphingobium herbicidovorans DSM 11019

Annotated modifications

640 mg/l Dichlorprop

add polyvinylalcohol (20 g/l) before autoclaving

Modified strains

Annotated modifications

20 g/l Polyvinylalcohol

after autoclaving add 1 ml/l ethylmethylketone

Modified strains

Pseudomonas fluorescens DSM 6308

Annotated modifications

1 ml/l Ethylmethylketone

Grow this degradative strain in minimal medium under conditions selective for the plasmid applying the required specific carbon source. Addition of 0.01% yeast extract plus 50µg/mL tryptophane to the medium enhances growth of this strain

Modified strains

Pseudomonas sp. DSM 3935

Annotated modifications

50 µg/mL Tryptophan
0.1 g/l Yeast extract

PH 5, with hexane (1 µl per ml)

Modified strains

Alkanibacter difficilis DSM 14804

Annotated modifications

5.0
1 ml/l Hexane

PH 7.5, with 100 mg/l yeast extract and 20 ml of a 1 M dichloroacetate or chloroethanol filter-sterilized stock solution

Modified strains

Pseudomonas fluorescens DSM 6757

Annotated modifications

100.0 mg/l Yeast extract
7.5

PH 7.5, with 100 mg/l yeast extract and 20 ml of a 1 M mono-, or dichloroacetate filter-sterilized stock solution

Modified strains

Caballeronia glathei DSM 6756

Annotated modifications

100.0 mg/l Yeast extract
7.5

PH 7.5, with 100 mg/l yeast extract and 20 mM sodium chloroacetate from a filter-sterilized 1 mol/l stock solution

Modified strains

Pseudomonas putida DSM 6755

Annotated modifications

100.0 mg/l Yeast extract
7.5
20 mM Sodium chloroacetate

Plus 0.02 g/l tryptophan plus 2 g/l hexamethylenetetramine (= urotropine = methenamin)

Modified strains

Brevundimonas diminuta DSM 17668

Annotated modifications

0.02 g/l Tryptophan
2 g/l Hexamethylenetetramine

Plus 0.1 g/l phenol

Modified strains

Acinetobacter sp. DSM 16482

Annotated modifications

0.1 g/l Phenol

Plus 0.6 g/l NaCl, plus 0.9 g/l K-sulfanilate

Modified strains

Novosphingobium resinovorum DSM 103995

Annotated modifications

0.6 g/l NaCl
0.9 g/l K-sulfanilate

Plus 0.75 g/l 2,5-dichlorobenzoate

Modified strains

Achromobacter sp. DSM 26587

Annotated modifications

0.75 g/l 2,5-dichlorobenzoate

Plus 1 g/l sodiumsulfate plus biphenyl, supplied via incubation atmosphere; incubation time 2-3 weeks

Modified strains

Pseudomonas putida DSM 28064

Annotated modifications

1 g/l Sodium sulfate

Plus 2 mM Na-cholate plus 1.0 g/l KNO3; anaerobic

Modified strains

Pseudomonas stutzeri DSM 103613

Annotated modifications

1.0 g/l KNO3
2 mM Na-cholate

Plus 5 g/l methanol and with dichloromethane supplied via incubation atmosphere

Modified strains

Albibacter helveticus DSM 22800

Annotated modifications

5.0 g/l Methanol

Plus eugenol via the incubation atmosphere

Modified strains

Pseudomonas sp. DSM 14291

Plus 0.3 g/l of each glucose, glycerol, Na-succinate and 0.1 g/l 2,4,5-T

Modified strains

Ochrobactrum anthropi DSM 6753

Annotated modifications

0.3 g/l Glucose
0.3 g/l Glycerol
0.3 g/l Succinate
0.1 g/l 2,4,5-Trichlorophenoxyacetic acid

plus 0.4 % poly-b-hydroxybutyrate

Modified strains

Paucimonas lemoignei DSM 7445

Plus 25mg/mL yeast extract; plus 50 µL n-hexane/mL

Modified strains

Thauera butanivorans DSM 2080

Annotated modifications

25 mg/mL Yeast extract
50 ml/l Hexane

Plus 334 mg/l carbazol. Dissolve carbazol in an acetone stock solution, add an appropriate volume to the sterile culturing vessel, let the acetone evaporate, then add the mineral medium and inoculate

Modified strains

Sphingobium yanoikuyae DSM 28039

Annotated modifications

334 mg/l Carbazol

Rehydrate and grow freeze dried cells in liquid medium 1. Subsequently, cultivation may be carried out in medium 457 with 50 mg/l yeast extract and 3-chloro-1-propanol, added to the incubation atmosphere.

Modified strains

Pseudomonas putida DSM 7189

Supplemented with 1 g/l p-hydroxybenzoic acid

Modified strains

Paenibacillus validus DSM 6390

Annotated modifications

1 g/l 4-Hydroxybenzoic acid

supplemented with benzene via incubation atmosphere

Modified strains

Supplemented with isopropylbenzene (cumol) via the gas phase

Modified strains

Pseudomonas fluorescens DSM 6608

Supplemented with naphthalene or 3-xylene via incubation atmosphere

Modified strains

Sphingobium yanoikuyae DSM 6900

Supplemented with naphthalene via incubation atmosphere. Do NOT use Erlenmeyer flasks with baffles.

Modified strains

Pseudomonas abietaniphila DSM 6506

Supplemented with styrene via incubation atmosphere

Modified strains

Rhodococcus rhodochrous DSM 6697

Supplemented with toluene via incubation atmosphere

Modified strains

Pseudomonas putida DSM 6413
Pseudomonas putida DSM 6899

supply 3-nitrotoluene via incubation atmosphere

Modified strains

Pseudomonas putida DSM 11738

With 0.05 g/l yeast extract (add methanol or 1,2-dichloroethane to the incubation atmosphere)

Modified strains

Ancylobacter rudongensis DSM 9000

Annotated modifications

0.05 g/l Yeast extract

With 0.05 g/l yeast extract, supply naphthalene via the incubation atmosphere

Modified strains

Pseudomonas vancouverensis DSM 8368

Annotated modifications

0.05 g/l Yeast extract

With 0.26 g/l taurine

Modified strains

Paracoccus pantotrophus DSM 15418

Annotated modifications

0.26 g/l Taurine

With 0.26 g/l taurine (added from a filter sterilized stock solution after autoclaving)

Modified strains

Comamonas sp. DSM 15091

Annotated modifications

0.26 g/l Taurine

With 0.26 g/l taurine (added from a filtersterilized stock solution after autoclaving)

Modified strains

Burkholderia sp. DSM 15090

Annotated modifications

0.26 g/l Taurine

With 0.3 g/l glucose, glycerol and Na-succinate, each and 66 mg/l pentachlorophenol

Modified strains

Sphingobium chlorophenolicum DSM 7098

Annotated modifications

0.3 g/l Glucose
0.3 g/l Glycerol
0.3 g/l Sodium succinate
66 mg/l Pentachlorophenol

With 0.3 g/l phenol

Modified strains

Acinetobacter sp. DSM 12734

Annotated modifications

0.3 g/l Phenol

With 0.4 g/l phenol

Modified strains

Pseudomonas putida DSM 12735

Annotated modifications

0.4 g/l Phenol

With 0.5 g/l 2,4-dichlorophenoxyacetate and 0.05 mg/l yeast extract

Modified strains

Paraburkholderia phenoliruptrix DSM 17773

Annotated modifications

0.5 g/l 2,4-dichlorophenoxyacetate
0.05 g/l Yeast extract

With 0.5 g/l 2-chlorobenzoate

Modified strains

Pseudomonas sp. DSM 9959

Annotated modifications

0.5 g/l 2-Chlorobenzoate

With 0.5 g/l 3-chlorosalicylic acid; Rehydrate and grow freeze-dried cells from ampoule in liquid medium 1 first. Subsequent subcultivation may be carried out in liquid medium or on agar plates and in medium 457 with 3-chlorosalicylate.

Modified strains

Sphingomonas sp. DSM 12401

Annotated modifications

0.5 g/l 3-Chlorosalicylic acid

With 0.5 g/l phenol

Modified strains

Alcaligenes faecalis subsp. phenolicus DSM 16503

Annotated modifications

0.5 g/l Phenol

With 0.5 ml/l Tween 20

Modified strains

Pseudomonas fluorescens DSM 9958

Annotated modifications

0.5 ml/l Tween 20

With 0.8 g/l sodium salicylate

Modified strains

Pseudomonas moorei DSM 12647

Annotated modifications

0.8 g/l Salicylate

With 1 g/l 3-chlorobenzoate

Modified strains

Pseudomonas knackmussii DSM 6978

Annotated modifications

1 g/l 3-Chlorobenzoate

With 1 g/l dimethoate from a filter sterilized stock solution

Modified strains

Arthrobacter sp. DSM 12418

Annotated modifications

1 g/l Dimethoate

With 1 g/l hexadecan and 20 mg yeast extract

Modified strains

Pseudomonas thermotolerans DSM 14292

Annotated modifications

1 g/l Hexadecane
20 mg/l Yeast extract

With 1 g/l Na-saccharin

Modified strains

Sphingobium xenophagum DSM 14677

Annotated modifications

1 g/l Na-saccharin

With 1 g/l sodium benzoate

Modified strains

Bacillus benzoevorans DSM 6410

Annotated modifications

1.0 g/l Sodium benzoate

With 1 g/l sodium benzoate; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 545. Subsequent subculturing may be carried out in liquid medium or on agar plates and under selective conditions (M. 457 plus benzoate)

Modified strains

Bacillus benzoevorans DSM 6385

Annotated modifications

1.0 g/l Sodium benzoate

With 1,2 g/l 4-phenolsulfonate; Rehydrate and grow freeze dried cells from ampoules in liquid medium 1. Subsequent subculturing may be carried out also in medium 457 or on agar plates.

Modified strains

Comamonas testosteroni DSM 14576

Annotated modifications

1.2 g/l 4-phenolsulfonate

With 1.68 g/l dibenzofuran

Modified strains

Janibacter terrae DSM 11213
Janibacter terrae DSM 11214
Janibacter terrae DSM 11215
Janibacter terrae DSM 11216
Janibacter terrae DSM 11217
Janibacter terrae DSM 11218
Janibacter terrae DSM 11219
Janibacter terrae DSM 11345
Janibacter terrae DSM 11346

Annotated modifications

1.68 g/l Dibenzofuran

With 100 mg/l 2,4-dinitrotoluene

Modified strains

Achromobacter sp. DSM 22896

Annotated modifications

100 mg/l 2,4-Dinitrotoluene

With 100 mg/l p-nitrophenol

Modified strains

Pseudomonas putida DSM 12448

Annotated modifications

100 mg/l 4-Nitrophenol

With 150 mg/l phenol from a filter sterilized stock solution and 50 mg/l yeast extract

Modified strains

Paraburkholderia kururiensis DSM 13646

Annotated modifications

150.0 mg/l Phenol
50 mg/l Yeast extract

With 2 g/l mercaptosuccinate

Modified strains

Variovorax paradoxus DSM 21786

Annotated modifications

2 g/l Mercaptosuccinate

With 2,4-dinitrotoluene as the sole source of carbon

Modified strains

Burkholderia sp. DSM 22944

With 2-chlorophenol via incubation atmosphere

Modified strains

Pseudomonas sp. DSM 13337

With 2.5 mM 4-chlorobenzoate

Modified strains

Pseudarthrobacter oxydans DSM 6612
Paraburkholderia sp. DSM 6613

Annotated modifications

2.5 mM 4-Chlorobenzoate

With 2.5 mM 4-hydroxy- or 4-fluorobenzoate

Modified strains

Pseudomonas sp. DSM 6611

Annotated modifications

2.5 mM 4-Hydroxybenzoic acid

With 2.5 mM 4-hydroxy- or fluorobenzoate

Modified strains

Paracoccus pantotrophus DSM 6610

Annotated modifications

2.5 mM 4-Hydroxybenzoic acid

With 300 mg/l 2,4-dichlorophenoxyacetate (2,4-D)

Modified strains

Paraburkholderia hospita DSM 17164

Annotated modifications

300 mg/l 2,4-dichlorophenoxyacetate

With 34 g/l NaCl and 10 ml/l ethylene glycol

Modified strains

Halomonas elongata DSM 6706

Annotated modifications

34.0 g/l NaCl
10 ml/l Ethylene glycol

With 50 mg/l phenol; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Pseudomonas sp. DSM 14157

Annotated modifications

50.0 mg/l Phenol

with 50 mg/l yeast extract (add 2,4-dimethylaniline to the incubation atmosphere)

Modified strains

Annotated modifications

50 mg/l Yeast extract

With 50 mg/l yeast extract (add N,N-dimethylaniline to the incubation atmosphere)

Modified strains

Sphingobium xenophagum DSM 8566

Annotated modifications

50.0 mg/l Yeast extract

With 50 mg/L yeast extract. Add naphthalene to the incubation atmosphere

Modified strains

Pseudomonas fluorescens DSM 8369

Annotated modifications

50 mg/L Yeast extract

With 8 mM aniline (dissolve 7.5 g/l in water, sterilize per filter and add 100 ml/l to the autoclaved medium)

Modified strains

Delftia sp. DSM 6411

Annotated modifications

8 mM Aniline

With biphenyl supplied via incubation atmosphere

Modified strains

Paraburkholderia xenovorans DSM 17367

With chlorobenzene via vapor phase

Modified strains

Pseudomonas veronii DSM 16272
Pseudomonas veronii DSM 16273
Pseudomonas fluorescens DSM 16274

With dibenzofuran

Modified strains

Janibacter terrae DSM 11220

With dibenzofuran via incubation atmosphere

Modified strains

Rhizorhabdus wittichii DSM 6014

With dibenzofuran, supplied via incubation atmosphere

Modified strains

Sphingobium aromaticiconvertens DSM 12677

With dichloromethane in the incubation atmosphere; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 162. Subsequent subculturing may be carried out in liquid medium or on agar plates.

Modified strains

Hyphomicrobium sp. DSM 6363

With naphthalene

Modified strains

Rhodococcus ruber DSM 7512

With pyrene which is sublimated onto inoculated agar plates of medium 457

Modified strains

Novosphingobium pentaromativorans DSM 17173

With styrene supplied via gasphase

Modified strains

Xanthobacter sp. DSM 6696

With styrene via incubation atmosphere (low amounts, toxic!)

Modified strains

Pseudomonas fluorescens DSM 6290

With tolene via incubation atmosphere

Modified strains

Pseudomonas veronii DSM 11567

With toluene supplied via the incubation atmosphere

Modified strains

Pseudomonas stutzeri DSM 24582

With toluene via incubation atmosphere

Modified strains

Pseudomonas fluorescens DSM 11531
Pseudomonas fluorescens DSM 11532
Pseudomonas sp. DSM 11533
Pseudomonas sp. DSM 11535
Pseudomonas sp. DSM 11562
Pseudomonas brenneri DSM 11563
Pseudomonas sp. DSM 11564
Pseudomonas sp. DSM 11565
Pseudomonas mandelii DSM 11566
Pseudomonas sp. DSM 11568
Pseudomonas sp. DSM 11569
Pseudomonas sp. DSM 11570
Pseudomonas sp. DSM 11591
Novosphingobium aromaticivorans DSM 12444
Novosphingobium stygium DSM 12445
Novosphingobium subterraneum DSM 12447
Sphingobium chungbukense DSM 16638

With vitamins (see medium 462, add dichlorobenzene to the incubation atmosphere)

Modified strains

Comamonas sp. DSM 6838

With yeast extract (50 mg/l) and 2-(4-sulfophenyl)butyrat (5 g/l) from a filter sterilized stock solution

Modified strains

Delftia acidovorans DSM 12586

Annotated modifications

50 mg/l Yeast extract
5 g/l 2-(4-sulfophenyl)butyrat

Without ammonium sulfate plus 0.2 g/l Na- succinate plus nitrobenzene supplied via the incubation atmosphere

Modified strains

Comamonas sp. DSM 22897

Annotated modifications

Ammonium Sulfate
0.2 g/l Sodium succinate

Without ammonium sulfate, with 2-nitrotoluene supplied via the incubation atmosphere

Modified strains

Acidovorax sp. DSM 22895

Annotated modifications

Ammonium Sulfate
Modifications
(add benzene to the incubation atmosphere) (add camphor to the incubation atmosphere) (add dibenzofurane to the incubation atmosphere) (add first benzene then 1,4-dichlorobenzene to the incubation atmosphere) (add isopropanol to the incubation atmosphere) (add methoxy-2-propanol to the incubation atmosphere) (add naphthalene to the incubation atmosphere) (add o-xylene to the incubation atmosphere) (add toluene to the incubation atmosphere) (after autoclaving add 10 ml/l of a neutralized filter-sterilized solution of 5 g/l 4-nitrophenol); Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 220. Subsequent subculturing may be carried out in liquid medium or on agar plates (after autoclaving add 10 mmol/l dichloromethane and/or methanol) (after autoclaving and cooling, add 1 g/l filter-sterilized trans-caryophyllene) (after autoclaving and cooling, add 1 ml/l filter-sterilized trans-caryophyllene) (after autoclaving, add 0.5 g/l aniline from a filter-sterilized stock solution) (after autoclaving, add 1 g/l chlorobenzoate from a filter-sterilized 10-fold concentrated stock solution) (pH 6, add 0,5 g/l of p-chloroaniline from a filter sterilized stock solution to the autoclaved medium) (pH 7.5, with 100 mg/l yeast extract. After autoclaving add 20 ml of a 1M dichloropropionate filter-sterilized stock solution) (supply 3-chlorotoluene via incubation atmosphere) (supply 4-chloroaniline via incubation atmosphere) (supply isopropylbenzene (cumol) via the gas phase) (supply naphthalene via gasphase) (supply o-xylene via incubation atmosphere) (supply p-cresol via the gas phase) (supply pseudocumene via incubation atmosphere) (supply toluene via the incubation atmosphere) (with 50 mg/l yeast extract, add 1,4-dichlorobenzene to the incubation atmosphere) add 0.2 g/l p-cresol add 1 g/l of chloro- or fluoroacetate from a neutralized and filter sterilized stock solution to the autoclaved medium add 1 mmol/l of 4-carboxybiphenyl ether from a filter sterilized stock solution to the autoclaved medium add 1,2,4-trichlorobenzene to the gas phase add 1,4-dichlorobenzene to the incubation atmosphere add 100 mg/L cetyltrimethylammonium chloride from a filter sterilized stock solution to the autoclaved medium add 2,5 mmol/l 4-sulfophthalate from a filter sterilized stock solution to the autoclaved medium add 2-chloropropenol to the incubation atmosphere add 300 mg/l 1-chloro-4-nitrobenzene to the autoclaved and cooled medium add 4 mM aniline from a filter sterilized stock solution to the autoclaved medium add 40 ml 4-chlorobenzenesulfonic acid from a 100 mmol/l filter-sterilized stock solution to 1 l autoclaved medium 457 add 5 mmol/l acetanilide from a filter sterilized stock solution (30 mmol/l) add 5 mmol/l acetanilide from a filter sterilized stock solution (30 mmol/l) to the autoclaved medium add 50 mg/l of yeast extract and some crystals of 3-chlorodibenzofuran. Transformation is indicated by deep yellow colour of medium. add 50 mg/l yeast extract and 1 g/l fluoroacetate from a filter sterilized stock solution to the autoclaved medium add 640 mg/l dichlorprop [2-(2,4-dichlorophenoxy)propionic acid] from a filtersterilized stock solution to the sterilized and cooled medium add polyvinylalcohol (20 g/l) before autoclaving after autoclaving add 1 ml/l ethylmethylketone Grow this degradative strain in minimal medium under conditions selective for the plasmid applying the required specific carbon source. Addition of 0.01% yeast extract plus 50µg/mL tryptophane to the medium enhances growth of this strain PH 5, with hexane (1 µl per ml) PH 7.5, with 100 mg/l yeast extract and 20 ml of a 1 M dichloroacetate or chloroethanol filter-sterilized stock solution PH 7.5, with 100 mg/l yeast extract and 20 ml of a 1 M mono-, or dichloroacetate filter-sterilized stock solution PH 7.5, with 100 mg/l yeast extract and 20 mM sodium chloroacetate from a filter-sterilized 1 mol/l stock solution Plus 0.02 g/l tryptophan plus 2 g/l hexamethylenetetramine (= urotropine = methenamin) Plus 0.1 g/l phenol Plus 0.6 g/l NaCl, plus 0.9 g/l K-sulfanilate Plus 0.75 g/l 2,5-dichlorobenzoate Plus 1 g/l sodiumsulfate plus biphenyl, supplied via incubation atmosphere; incubation time 2-3 weeks Plus 2 mM Na-cholate plus 1.0 g/l KNO3; anaerobic Plus 5 g/l methanol and with dichloromethane supplied via incubation atmosphere Plus eugenol via the incubation atmosphere Plus 0.3 g/l of each glucose, glycerol, Na-succinate and 0.1 g/l 2,4,5-T plus 0.4 % poly-b-hydroxybutyrate Plus 25mg/mL yeast extract; plus 50 µL n-hexane/mL Plus 334 mg/l carbazol. Dissolve carbazol in an acetone stock solution, add an appropriate volume to the sterile culturing vessel, let the acetone evaporate, then add the mineral medium and inoculate Rehydrate and grow freeze dried cells in liquid medium 1. Subsequently, cultivation may be carried out in medium 457 with 50 mg/l yeast extract and 3-chloro-1-propanol, added to the incubation atmosphere. Supplemented with 1 g/l p-hydroxybenzoic acid supplemented with benzene via incubation atmosphere Supplemented with isopropylbenzene (cumol) via the gas phase Supplemented with naphthalene or 3-xylene via incubation atmosphere Supplemented with naphthalene via incubation atmosphere. Do NOT use Erlenmeyer flasks with baffles. Supplemented with styrene via incubation atmosphere Supplemented with toluene via incubation atmosphere supply 3-nitrotoluene via incubation atmosphere With 0.05 g/l yeast extract (add methanol or 1,2-dichloroethane to the incubation atmosphere) With 0.05 g/l yeast extract, supply naphthalene via the incubation atmosphere With 0.26 g/l taurine With 0.26 g/l taurine (added from a filter sterilized stock solution after autoclaving) With 0.26 g/l taurine (added from a filtersterilized stock solution after autoclaving) With 0.3 g/l glucose, glycerol and Na-succinate, each and 66 mg/l pentachlorophenol With 0.3 g/l phenol With 0.4 g/l phenol With 0.5 g/l 2,4-dichlorophenoxyacetate and 0.05 mg/l yeast extract With 0.5 g/l 2-chlorobenzoate With 0.5 g/l 3-chlorosalicylic acid; Rehydrate and grow freeze-dried cells from ampoule in liquid medium 1 first. Subsequent subcultivation may be carried out in liquid medium or on agar plates and in medium 457 with 3-chlorosalicylate. With 0.5 g/l phenol With 0.5 ml/l Tween 20 With 0.8 g/l sodium salicylate With 1 g/l 3-chlorobenzoate With 1 g/l dimethoate from a filter sterilized stock solution With 1 g/l hexadecan and 20 mg yeast extract With 1 g/l Na-saccharin With 1 g/l sodium benzoate With 1 g/l sodium benzoate; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 545. Subsequent subculturing may be carried out in liquid medium or on agar plates and under selective conditions (M. 457 plus benzoate) With 1,2 g/l 4-phenolsulfonate; Rehydrate and grow freeze dried cells from ampoules in liquid medium 1. Subsequent subculturing may be carried out also in medium 457 or on agar plates. With 1.68 g/l dibenzofuran With 100 mg/l 2,4-dinitrotoluene With 100 mg/l p-nitrophenol With 150 mg/l phenol from a filter sterilized stock solution and 50 mg/l yeast extract With 2 g/l mercaptosuccinate With 2,4-dinitrotoluene as the sole source of carbon With 2-chlorophenol via incubation atmosphere With 2.5 mM 4-chlorobenzoate With 2.5 mM 4-hydroxy- or 4-fluorobenzoate With 2.5 mM 4-hydroxy- or fluorobenzoate With 300 mg/l 2,4-dichlorophenoxyacetate (2,4-D) With 34 g/l NaCl and 10 ml/l ethylene glycol With 50 mg/l phenol; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates with 50 mg/l yeast extract (add 2,4-dimethylaniline to the incubation atmosphere) With 50 mg/l yeast extract (add N,N-dimethylaniline to the incubation atmosphere) With 50 mg/L yeast extract. Add naphthalene to the incubation atmosphere With 8 mM aniline (dissolve 7.5 g/l in water, sterilize per filter and add 100 ml/l to the autoclaved medium) With biphenyl supplied via incubation atmosphere With chlorobenzene via vapor phase With dibenzofuran With dibenzofuran via incubation atmosphere With dibenzofuran, supplied via incubation atmosphere With dichloromethane in the incubation atmosphere; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 162. Subsequent subculturing may be carried out in liquid medium or on agar plates. With naphthalene With pyrene which is sublimated onto inoculated agar plates of medium 457 With styrene supplied via gasphase With styrene via incubation atmosphere (low amounts, toxic!) With tolene via incubation atmosphere With toluene supplied via the incubation atmosphere With toluene via incubation atmosphere With vitamins (see medium 462, add dichlorobenzene to the incubation atmosphere) With yeast extract (50 mg/l) and 2-(4-sulfophenyl)butyrat (5 g/l) from a filter sterilized stock solution Without ammonium sulfate plus 0.2 g/l Na- succinate plus nitrobenzene supplied via the incubation atmosphere Without ammonium sulfate, with 2-nitrotoluene supplied via the incubation atmosphere