Strain modifications for Medium 457

(add benzene to the incubation atmosphere)

(add camphor to the incubation atmosphere)

(add dibenzofurane to the incubation atmosphere)

(add first benzene then 1,4-dichlorobenzene to the incubation atmosphere)

(add isopropanol to the incubation atmosphere)

(add methoxy-2-propanol to the incubation atmosphere)

(add naphthalene to the incubation atmosphere)

(add o-xylene to the incubation atmosphere)

(add toluene to the incubation atmosphere)

(after autoclaving add 10 ml/l of a neutralized filter-sterilized solution of 5 g/l 4-nitrophenol); Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 220. Subsequent subculturing may be carried out in liquid medium or on agar plates

(after autoclaving add 10 mmol/l dichloromethane and/or methanol)

(after autoclaving and cooling, add 1 g/l filter-sterilized trans-caryophyllene)

(after autoclaving and cooling, add 1 ml/l filter-sterilized trans-caryophyllene)

(after autoclaving, add 0.5 g/l aniline from a filter-sterilized stock solution)

(after autoclaving, add 1 g/l chlorobenzoate from a filter-sterilized 10-fold concentrated stock solution)

(pH 6, add 0,5 g/l of p-chloroaniline from a filter sterilized stock solution to the autoclaved medium)

(pH 7.5, with 100 mg/l yeast extract. After autoclaving add 20 ml of a 1M dichloropropionate filter-sterilized stock solution)

(supply 3-chlorotoluene via incubation atmosphere)

(supply 4-chloroaniline via incubation atmosphere)

(supply isopropylbenzene (cumol) via the gas phase)

(supply naphthalene via gasphase)

(supply o-xylene via incubation atmosphere)

(supply p-cresol via the gas phase)

(supply pseudocumene via incubation atmosphere)

(supply toluene via the incubation atmosphere)

(with 50 mg/l yeast extract, add 1,4-dichlorobenzene to the incubation atmosphere)

add 0.2 g/l p-cresol

add 1 g/l of chloro- or fluoroacetate from a neutralized and filter sterilized stock solution to the autoclaved medium

add 1 mmol/l of 4-carboxybiphenyl ether from a filter sterilized stock solution to the autoclaved medium

add 1,2,4-trichlorobenzene to the gas phase

add 1,4-dichlorobenzene to the incubation atmosphere

add 100 mg/L cetyltrimethylammonium chloride from a filter sterilized stock solution to the autoclaved medium

add 2,5 mmol/l 4-sulfophthalate from a filter sterilized stock solution to the autoclaved medium

add 2-chloropropenol to the incubation atmosphere

add 300 mg/l 1-chloro-4-nitrobenzene to the autoclaved and cooled medium

add 4 mM aniline from a filter sterilized stock solution to the autoclaved medium

add 40 ml 4-chlorobenzenesulfonic acid from a 100 mmol/l filter-sterilized stock solution to 1 l autoclaved medium 457

add 5 mmol/l acetanilide from a filter sterilized stock solution (30 mmol/l)

add 5 mmol/l acetanilide from a filter sterilized stock solution (30 mmol/l) to the autoclaved medium

add 50 mg/l of yeast extract and some crystals of 3-chlorodibenzofuran. Transformation is indicated by deep yellow colour of medium.

add 50 mg/l yeast extract and 1 g/l fluoroacetate from a filter sterilized stock solution to the autoclaved medium

add 640 mg/l dichlorprop [2-(2,4-dichlorophenoxy)propionic acid] from a filtersterilized stock solution to the sterilized and cooled medium

add polyvinylalcohol (20 g/l) before autoclaving

after autoclaving add 1 ml/l ethylmethylketone

Grow this degradative strain in minimal medium under conditions selective for the plasmid applying the required specific carbon source. Addition of 0.01% yeast extract plus 50µg/mL tryptophane to the medium enhances growth of this strain

PH 5, with hexane (1 µl per ml)

PH 7.5, with 100 mg/l yeast extract and 20 ml of a 1 M dichloroacetate or chloroethanol filter-sterilized stock solution

PH 7.5, with 100 mg/l yeast extract and 20 ml of a 1 M mono-, or dichloroacetate filter-sterilized stock solution

PH 7.5, with 100 mg/l yeast extract and 20 mM sodium chloroacetate from a filter-sterilized 1 mol/l stock solution

Plus 0.02 g/l tryptophan plus 2 g/l hexamethylenetetramine (= urotropine = methenamin)

Plus 0.1 g/l phenol

Plus 0.6 g/l NaCl, plus 0.9 g/l K-sulfanilate

Plus 0.75 g/l 2,5-dichlorobenzoate

Plus 1 g/l sodiumsulfate plus biphenyl, supplied via incubation atmosphere; incubation time 2-3 weeks

Plus 2 mM Na-cholate plus 1.0 g/l KNO₃; anaerobic

Plus 5 g/l methanol and with dichloromethane supplied via incubation atmosphere

Plus eugenol via the incubation atmosphere

Plus 0.3 g/l of each glucose, glycerol, Na-succinate and 0.1 g/l 2,4,5-T

plus 0.4 % poly-b-hydroxybutyrate

Plus 25mg/mL yeast extract; plus 50 µL n-hexane/mL

Plus 334 mg/l carbazol. Dissolve carbazol in an acetone stock solution, add an appropriate volume to the sterile culturing vessel, let the acetone evaporate, then add the mineral medium and inoculate

Rehydrate and grow freeze dried cells in liquid medium 1. Subsequently, cultivation may be carried out in medium 457 with 50 mg/l yeast extract and 3-chloro-1-propanol, added to the incubation atmosphere.

Supplemented with 1 g/l p-hydroxybenzoic acid

supplemented with benzene via incubation atmosphere

Supplemented with isopropylbenzene (cumol) via the gas phase

Supplemented with naphthalene or 3-xylene via incubation atmosphere

Supplemented with naphthalene via incubation atmosphere. Do NOT use Erlenmeyer flasks with baffles.

Supplemented with styrene via incubation atmosphere

Supplemented with toluene via incubation atmosphere

supply 3-nitrotoluene via incubation atmosphere

With 0.05 g/l yeast extract (add methanol or 1,2-dichloroethane to the incubation atmosphere)

With 0.05 g/l yeast extract, supply naphthalene via the incubation atmosphere

With 0.26 g/l taurine

With 0.26 g/l taurine (added from a filter sterilized stock solution after autoclaving)

With 0.26 g/l taurine (added from a filtersterilized stock solution after autoclaving)

With 0.3 g/l glucose, glycerol and Na-succinate, each and 66 mg/l pentachlorophenol

With 0.3 g/l phenol

With 0.4 g/l phenol

With 0.5 g/l 2,4-dichlorophenoxyacetate and 0.05 mg/l yeast extract

With 0.5 g/l 2-chlorobenzoate

With 0.5 g/l 3-chlorosalicylic acid; Rehydrate and grow freeze-dried cells from ampoule in liquid medium 1 first. Subsequent subcultivation may be carried out in liquid medium or on agar plates and in medium 457 with 3-chlorosalicylate.

With 0.5 g/l phenol

With 0.5 ml/l Tween 20

With 0.8 g/l sodium salicylate

With 1 g/l 3-chlorobenzoate

With 1 g/l dimethoate from a filter sterilized stock solution

With 1 g/l hexadecan and 20 mg yeast extract

With 1 g/l Na-saccharin

With 1 g/l sodium benzoate

With 1 g/l sodium benzoate; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 545. Subsequent subculturing may be carried out in liquid medium or on agar plates and under selective conditions (M. 457 plus benzoate)

With 1,2 g/l 4-phenolsulfonate; Rehydrate and grow freeze dried cells from ampoules in liquid medium 1. Subsequent subculturing may be carried out also in medium 457 or on agar plates.

With 1.68 g/l dibenzofuran

With 100 mg/l 2,4-dinitrotoluene

With 100 mg/l p-nitrophenol

With 150 mg/l phenol from a filter sterilized stock solution and 50 mg/l yeast extract

With 2 g/l mercaptosuccinate

With 2,4-dinitrotoluene as the sole source of carbon

With 2-chlorophenol via incubation atmosphere

With 2.5 mM 4-chlorobenzoate

With 2.5 mM 4-hydroxy- or 4-fluorobenzoate

With 2.5 mM 4-hydroxy- or fluorobenzoate

With 300 mg/l 2,4-dichlorophenoxyacetate (2,4-D)

With 34 g/l NaCl and 10 ml/l ethylene glycol

With 50 mg/l phenol; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates

with 50 mg/l yeast extract (add 2,4-dimethylaniline to the incubation atmosphere)

With 50 mg/l yeast extract (add N,N-dimethylaniline to the incubation atmosphere)

With 50 mg/L yeast extract. Add naphthalene to the incubation atmosphere

With 8 mM aniline (dissolve 7.5 g/l in water, sterilize per filter and add 100 ml/l to the autoclaved medium)

With biphenyl supplied via incubation atmosphere

With chlorobenzene via vapor phase

With dibenzofuran

With dibenzofuran via incubation atmosphere

With dibenzofuran, supplied via incubation atmosphere

With dichloromethane in the incubation atmosphere; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 162. Subsequent subculturing may be carried out in liquid medium or on agar plates.

With naphthalene

With pyrene which is sublimated onto inoculated agar plates of medium 457

With styrene supplied via gasphase

With styrene via incubation atmosphere (low amounts, toxic!)

With tolene via incubation atmosphere

With toluene supplied via the incubation atmosphere

With toluene via incubation atmosphere

With vitamins (see medium 462, add dichlorobenzene to the incubation atmosphere)

With yeast extract (50 mg/l) and 2-(4-sulfophenyl)butyrat (5 g/l) from a filter sterilized stock solution

Without ammonium sulfate plus 0.2 g/l Na- succinate plus nitrobenzene supplied via the incubation atmosphere

Without ammonium sulfate, with 2-nitrotoluene supplied via the incubation atmosphere