Strain modifications for Medium 462

(add 2 mmol/l of N-ethylacetanilide from a filter sterilized stock solution to the autoclaved medium)

(add 250 µl/l sterile 1,2,4-trichlorobenzene to the autoclaved and cooled medium)

(after autoclaving add 1 g/l filter-sterilized 1-chlorohexane)

(supply toluene via incubation atmosphere)

(with hexane supplied via incubation atmosphere)

Add 0.5 g/l umbelliferon before sterilizing

Plus 0.2 g/l 2-hydroxypyridine

Plus 0.4 g/l vanillin

Plus 0.5 g/l vanillin

Plus 1 g/l sodium dodecyl sulfate (SDS)

Plus 1.0 g/l technical nonylphenol. Stirr liquified agar samples intended for plates or slants heavily to obtain a suspension. Take care to adjust pH to 7.2.

Plus 100 mg/l methyl-parathion, supplied from an acetone stock solution into the dry sterile culturing vessel. Let evaporate the acetone, fill in the mineral medium under sterile conditions

Plus 2g/l methanol and dichlormethane via atmosphere

Plus 4-fluorobenzoic acid (0.2 g/l)

Plus 6 mM p-toluenesulfonate

Plus biphenyl supplied via the incubation atmosphere

Plus styrene supplied via incubation atmosphere

Plus 0,4g/L Antipyrin

Plus 0,5g/L 2,4-Dichlorphenoxyessigsäure

plus 0.25% MeOH. Must be precultured in medium 1 plus MeOH (not in medium 464) to grow in medium 462 plus MeOH.

plus 10 mM canavanine

plus 3-chlorotoluene via incubation atmosphere

plus 5 mM 3-chlorobenzenesulfonate

plus 50 µM alpha-hexachlorocyclohexane, supplied to vials via an acetone solution. Let acetone evaporate before dispensing the mineral medium.

Plus 6 mM 4-toluenesulfonate

plus 600 mg/L BTEX mixture (benzene, toluene, ethylbenzene, and o-, m-, p-xylene, v/v 1:1:1:1:1:1) plus 0.5 g/L yeast extract

Salt content adapted to seawater content plus phenanthrene which is sublimated onto the inoculated agar plates

With 0.01 g/l yeast extract (supply styrene via gas phase)

With 0.4 g/L 2-chlorobenzoate and 0.4 g/L 3-chlorobenzoate

With 1-chlorohexan via incubation atmosphere

With 1.93 g/l 4-hydroxybenzoate

With 5 g/L methanol, dichloromethane may be added to the incubation atmosphere

With dichloromethane, supplied via incubation atmosphere; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1 with methanol. Subsequent subculturing may be carried out in liquid medium or on agar plates.

With nonylphenol directly applied to the medium (e.g. one drop to 20 ml)

with phenanthrene via the incubation atmosphere

With phenanthrene which is sublimated onto inoculated agar plates of medium 462

With styrene via incubation atmosphere