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DSMZ

Strain modifications for Medium 503

27 distinct modifications were found.

Add 1.70 g/l NaNO3 to solution A and replace D-glucose with 1.25 g/l taurine as substrate.

Modified strains

Castellaniella denitrificans DSM 11046

Annotated modifications

1.70 g NaNO3
to Solution A
1.25 g Taurine
to Solution C
D-Glucose
to Solution C

Omit glucose and sodium resazurin. Supplement medium with 2.20 g/l sodium phosphite hydrate.

Modified strains

Phosphitispora fastidiosa DSM 112739

Annotated modifications

Sodium resazurin
D-Glucose
to Solution C
2.20 g Na2HPO3 x 5 H2O
to Solution C

Omit glucose. Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 25 - 50 mg sodium dithionite per liter (e.g. from a freshly prepared filter-sterilized 5% w/v solution) for reduction of the medium prior to inoculation.

Modified strains

Syntrophobacter pfennigii DSM 10092

Annotated modifications

+ 1 ml Na-dithionite solution (5% w/v)
0.70 g Na2SO4
to Solution A
D-Glucose
to Solution C
1.50 g Na-propionate
to Solution C
0.10 g Na2S x 9 H2O
to Solution E
Na2S x 9 H2O
to Solution E

Omit glucose. Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration.

Modified strains

Thermoanaerobaculum aquaticum DSM 24856

Annotated modifications

D-Glucose
0.10 g Yeast extract
to Solution A
0.85 g NaNO3
to Solution A
1.10 g Na-pyruvate
to Solution C

Omit sodium carbonate, reduce amount of D-glucose to 2.00 g/l and add 1.00 g/l yeast extract as substrate. Adjust pH of complete medium to 5.5.

Modified strains

Lucifera butyrica DSM 27520

Annotated modifications

5.5
- Solution B
1.00 g Yeast extract
to Solution A
2.00 g D-Glucose
to Solution C
D-Glucose
to Solution C

Omit solution C. Use as final concentration: 10 mM taurine, 60 mM Na-formate, and 200 µM 1,4-naphthochinone as substrates added from anoxic stock solutions sterilized by filtration

Modified strains

Bilophila wadsworthia DSM 11045

Annotated modifications

10.0 mM Taurine
60.0 mM Na-formate
- Solution C
200 µm 1,4-Naphthoquinone

Reduce amount of D-glucose to 0.40 g/l and add 1.00 g/l yeast extract as substrate.

Modified strains

Anaerosolibacter carboniphilus DSM 103526

Annotated modifications

1.00 g Yeast extract
to Solution A
0.40 g D-Glucose
to Solution C
D-Glucose
to Solution C

Reduce amount of D-glucose to 1.80 g/l.

Modified strains

DSM 11480
DSM 12595

Annotated modifications

1.80 g D-Glucose
to Solution C
D-Glucose
to Solution C

Reduce amount of glucose to 2 mM (0.36 g/l); defined coculture with Methanospirillum hungatei. Incubate in the dark. Anaerobic

Modified strains

Mesobacillus stamsii DSM 19598

Annotated modifications

0.36 g D-Glucose
to Solution C
D-Glucose
to Solution C

Reduce glucose to 3g/l. Add 1g/l yeast extract.

Modified strains

Lentimicrobium saccharophilum DSM 100618

Annotated modifications

1 g Yeast extract
to Solution A
D-Glucose
to Solution C
3.00 g D-Glucose
to Solution C

Replace D-glucose with 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates added from anoxic stock solutions sterilized by filtration.

Modified strains

Propionivibrio pelophilus DSM 12018

Annotated modifications

0.20 g Yeast extract
to Solution A
D-Glucose
to Solution C
2.66 g L-Aspartic acid
to Solution C

Replace D-glucose with 0.50 g/l Na-pyruvate as substrate added from an anoxic stock solution sterilized by filtration. Supplement solution A with 2.0 g/l yeast extract.

Modified strains

Aminivibrio pyruvatiphilus DSM 25964

Annotated modifications

2.0 g Yeast extract
to Solution A
0.50 g Na-pyruvate
to Solution C
D-Glucose
to Solution C

Replace D-glucose with 0.50 g/l yeast extract and 1.00 g/l L-rhamnose as substrates added from anoxic stock solutions sterilized by filtration .

Modified strains

Pelorhabdus rhamnosifermentans DSM 111565

Annotated modifications

0.50 g Yeast extract
to Solution A
D-Glucose
to Solution C
1.00 g L-Rhamnose
to Solution C

Replace D-glucose with 0.82 g/l Na acetate and 3.20 g/l Na2 fumarate as substrates added from anoxic stock solutions sterilized by filtration.

Modified strains

Geovibrio thiophilus DSM 11263
Geobacter sp. DSM 11489

Annotated modifications

0.82 g Na acetate
to Solution A
D-Glucose
to Solution C
3.20 g Sodium fumarate
to Solution C

Replace D-glucose with 1.00 g/l yeast extract and 2.00 g/l Na glycolate as substrates.

Modified strains

Clostridium sp. DSM 11261

Annotated modifications

1.00 g Yeast extract
to Solution A
2.00 g Sodium glycolate
to Solution C
D-Glucose
to Solution C

Replace D-glucose with 1.00 g/l yeast extract and 2.50 g/l Na (D/L)-3 hydroxybutyrate as substrates.

Modified strains

DSM 11262

Annotated modifications

1.00 g Yeast extract
to Solution A
D-Glucose
to Solution C
2.50 g Na-3-hydroxybutyrate
to Solution C

Replace D-glucose with 1.00 g/l yeast extract and 5.00 g/l D-fructose as substrates added from anoxic stock solutions sterilized by filtration.

Modified strains

Propionispora vibrioides DSM 13305

Annotated modifications

1.00 g Yeast extract
to Solution A
D-Glucose
to Solution C
5.00 g D-Fructose
to Solution C

Replace D-glucose with 1.50 g/l of Na (D/L) 3 hydroxybutyrate or 2.00 g/l D-fructose as substrate.

Modified strains

Clostridium homopropionicum DSM 5847

Annotated modifications

D-Glucose
to Solution C
1.50 g Na-3-hydroxybutyrate (or 2.00 g D-fructose)
to Solution C

Replace D-glucose with 1.60 g/l Na2-maleate as substrate added from an anoxic stock solution sterilzed by filtration. Adjust pH of complete medium to 6.7 - 6.8.

Modified strains

Propionivibrio dicarboxylicus DSM 5885

Annotated modifications

6.7 - 6.8
1.60 g Sodium maleate
to Solution C
D-Glucose
to Solution C

Replace D-glucose with 2.00 g/l of xylose or xylan as substrate.

Modified strains

Breznakibacter xylanolyticus DSM 6779

Annotated modifications

2.00 g D-Xylose (or xylan)
to Solution C
D-Glucose
to Solution C

Replace D-glucose with 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone (BD BBL) as substrates.

Modified strains

Clostridium tunisiense DSM 15206

Annotated modifications

2.00 g Yeast extract
to Solution A
2.00 g Trypticase peptone
to Solution C
D-Glucose
to Solution C

Replace D-glucose with 2.50 g/l of Na2 succinate as substrate.

Modified strains

Propionivibrio sp. DSM 5849

Annotated modifications

2.50 g Sodium succinate
to Solution C
D-Glucose
to Solution C

Replace D-glucose with 2.50 g/l taurine as substrate.

Modified strains

Desulfonispora thiosulfatigenes DSM 11270

Annotated modifications

2.50 g Taurine
to Solution C
D-Glucose
to Solution C

Supplement medium after autoclaving with 0.1 g/l yeast extract, 2.0 g/l D-mannose, and 1 ml/l Wolin's vitamin solution (10x) added from anoxic stock solutions sterilized by filtration. Omit D-Glucose.

Modified strains

DSM 27582

Annotated modifications

0.1 g Yeast extract
to Solution A
D-Glucose
to Solution C
2.0 g D-Mannose
to Solution C
+ 1 ml Wolin's vitamin solution (10x)
to Solution D

Supplement medium after autoclaving with 0.50 g/l yeast extract and 0.40 g/l L-cysteine HCl x H2O added from sterile anoxic stock solutions.

Modified strains

Thermospira aquatica DSM 101182

Annotated modifications

0.50 g Yeast extract
0.40 g L-Cysteine HCl x H2O

Supplement medium with 2.00 g/l yeast extract added from an anoxic stock solution sterilized by filtration.

Modified strains

Opitutus sp. DSM 14424
Fusibacter fontis DSM 28450
Rectinema cohabitans DSM 100378
Petroclostridium xylanilyticum DSM 103557
Rhabdanaerobium thermarum DSM 105239

Annotated modifications

2.00 g Yeast extract
to Solution A

Supplement medium with 20 ml/l FeSO4 x 7 H2O solution (0.1% w/v), 1 ml/l Na-dithionite solution (5% w/v), 0.8 ml/l Methanol, and 2 g/l Trimethylamine-HCl. Omit D-Glucose.

Modified strains

Methanomethylovorans hollandica DSM 15978
Methanomethylovorans uponensis DSM 27305

Annotated modifications

+ 1 ml Na-dithionite solution (5% w/v)
+ 20 ml FeSO4 x 7 H2O solution (0.1% w/v)
D-Glucose
to Solution C
2 g Trimethylamine-HCl
to Solution C
0.8 ml Methanol
to Solution C
Modifications
Add 1.70 g/l NaNO3 to solution A and replace D-glucose with 1.25 g/l taurine as substrate. Omit glucose and sodium resazurin. Supplement medium with 2.20 g/l sodium phosphite hydrate. Omit glucose. Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 25 - 50 mg sodium dithionite per liter (e.g. from a freshly prepared filter-sterilized 5% w/v solution) for reduction of the medium prior to inoculation. Omit glucose. Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. Omit sodium carbonate, reduce amount of D-glucose to 2.00 g/l and add 1.00 g/l yeast extract as substrate. Adjust pH of complete medium to 5.5. Omit solution C. Use as final concentration: 10 mM taurine, 60 mM Na-formate, and 200 µM 1,4-naphthochinone as substrates added from anoxic stock solutions sterilized by filtration Reduce amount of D-glucose to 0.40 g/l and add 1.00 g/l yeast extract as substrate. Reduce amount of D-glucose to 1.80 g/l. Reduce amount of glucose to 2 mM (0.36 g/l); defined coculture with Methanospirillum hungatei. Incubate in the dark. Anaerobic Reduce glucose to 3g/l. Add 1g/l yeast extract. Replace D-glucose with 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates added from anoxic stock solutions sterilized by filtration. Replace D-glucose with 0.50 g/l Na-pyruvate as substrate added from an anoxic stock solution sterilized by filtration. Supplement solution A with 2.0 g/l yeast extract. Replace D-glucose with 0.50 g/l yeast extract and 1.00 g/l L-rhamnose as substrates added from anoxic stock solutions sterilized by filtration . Replace D-glucose with 0.82 g/l Na acetate and 3.20 g/l Na2 fumarate as substrates added from anoxic stock solutions sterilized by filtration. Replace D-glucose with 1.00 g/l yeast extract and 2.00 g/l Na glycolate as substrates. Replace D-glucose with 1.00 g/l yeast extract and 2.50 g/l Na (D/L)-3 hydroxybutyrate as substrates. Replace D-glucose with 1.00 g/l yeast extract and 5.00 g/l D-fructose as substrates added from anoxic stock solutions sterilized by filtration. Replace D-glucose with 1.50 g/l of Na (D/L) 3 hydroxybutyrate or 2.00 g/l D-fructose as substrate. Replace D-glucose with 1.60 g/l Na2-maleate as substrate added from an anoxic stock solution sterilzed by filtration. Adjust pH of complete medium to 6.7 - 6.8. Replace D-glucose with 2.00 g/l of xylose or xylan as substrate. Replace D-glucose with 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone (BD BBL) as substrates. Replace D-glucose with 2.50 g/l of Na2 succinate as substrate. Replace D-glucose with 2.50 g/l taurine as substrate. Supplement medium after autoclaving with 0.1 g/l yeast extract, 2.0 g/l D-mannose, and 1 ml/l Wolin's vitamin solution (10x) added from anoxic stock solutions sterilized by filtration. Omit D-Glucose. Supplement medium after autoclaving with 0.50 g/l yeast extract and 0.40 g/l L-cysteine HCl x H2O added from sterile anoxic stock solutions. Supplement medium with 2.00 g/l yeast extract added from an anoxic stock solution sterilized by filtration. Supplement medium with 20 ml/l FeSO4 x 7 H2O solution (0.1% w/v), 1 ml/l Na-dithionite solution (5% w/v), 0.8 ml/l Methanol, and 2 g/l Trimethylamine-HCl. Omit D-Glucose.