Strain modifications for Medium 535
40 distinct modifications were found.
(this medium is recommended for the routine cultivation of the strain)
1:10 diluted
By choice with 5% (v/v) blood
By choice with 5% (v/v) blood; obligately anaerobic
Culture on agar plates are extemely transparent, may be overlooked easily
For fluorescence; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates
Microaerophilic under 93% N2, 5% CO2, 2% O2
Plates; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates
Plus 0.2% Tween
Plus 0.2% Tween 80
Plus 10 mg MnSO4 plus yeast 0.5%
Plus 20% horse serum
Plus 20% horse serum; anaerobic
Plus 20% horse serum; microaerophilic
Plus 5% Blood
Plus MnSO4, pH 8.5
plus 0.2% Tween
plus 20% horse serum
Plus 5% blood; microaerophil
Rehydrate and grow freeze dried cells from ampoules in liquid medium. Subsequent subculturing may be carried out in liquid medium or on agar plates.
Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1 or 535. Subsequent subculturing may be carried out in liquid medium or on agar plates
Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 545. Subsequent subculturing may be carried out in liquid medium 545 or on agar plates
Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth. Subsequent subculturing may be carried out in liquid medium or on agar plates
Resuspend and grow freeze dried cells in liquid medium. Subsequent subculturing may be carried out on plates or in liquid medium.
With 0.2% Tween 80
With 5% (v/v) blood
With 5% blood by choice
With 5% defibrinated sheep blood
With 50% soil extract
Without agar
PH 5.5 - pH 6