1
Autoclave solution A without the vitamin solution. Autoclave solution B in half full, closed bottles. Autoclave solution C. Combine the sterile solutions A, B, and C after cooling. Aseptically add 1.0 ml of the vitamin solution (filter sterilized) to 1 litre of the sterile medium.
2
Adjust to pH 9.8. The medium may be solidified by adding 20 g/l agar, but care should be taken that the temperature of the solutions to be mixed are about 50°C, otherwise the agar will darken and degrade.
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