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Main sol. 26

Internal Solution-ID: S3283

Equipment needed: Tungsten lamp Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.20 g 0.2 -
2 1.00 g 1 6.171
3 0.50 g 0.5 6.487
4
(0.1% in H2O)
5.00 ml - -
5 0.50 g 0.5 3.674
6 0.40 g 0.4 1.623
7 0.40 g 0.4 6.844
8 0.40 g 0.4 7.478
9 0.05 g 0.05 0.34
10
(10 mg in 100 ml H2O)
0.40 ml 1.08 0.684
12 Trace element solution SL-6 1.00 ml - -
13 0.30 g 0.3 1.903
14
(0,1%)
0.50 ml - -
15 1000.00 ml 998.207 55408.8
Instructions:
  1. Use medium 27 and reduce the yeast extract concentration to 0.2 g/l. Adjust pH to 5.7. Incubate in the light using a tungsten lamp.
  2. Boil the medium for a few minutes. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121 C for 15 min. Sterile syringes are used to inoculate and remove samples.
26 ACID RHODOSPIRILLACEAE MEDIUM