Main sol. 1602
Internal Solution-ID: S3339
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.00 | g | 20 | 342.231 | |
| CaCl2 x 2 H2O | 0.45 | g | 0.45 | 3.061 | |
| Yeast extract | 0.33 | g | 0.33 | - | |
| Casitone | 1.00 | g | 1 | - | |
| Sea water salts solution | 1000.00 | ml | - | - | |
|
Cyanocobalamine
(vitamin B12) |
0.50 | mg | 5.0e-4 | 3.7e-4 | |
| 1 Adjust pH to 7.5 with 1M NaOH, autoclave at 121°C for 20 min. Add cyanocobalamine from a filter sterilized solution after autoclaving and cooling. | |||||
Media with this solution
| 1602 | CY/3-SEAWATER MEDIUM |